肺动脉高压大鼠肺动脉平滑肌细胞Ryanodine受体亚型的变化  

Change of ryanodine receptor subtypes expression in pulmonary artery smooth muscle cell from pulmonary hypertensive rats

作　　者：王颖[1] 李智[1] 刘立宾 丛华[1] 

机构地区：[1]中国医科大学药理学教研室,沈阳110001

出　　处：《中国药理学通报》2000年第6期628-630,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金!资助课题 ;No 39870 891

摘　　要：目的　查明大鼠肺动脉平滑肌细胞 (PASMC)上Ryanodine受体 (RyR)的亚型并检测其在肺动脉高压 (PAH)时表达水平的变化。方法　采用大鼠一次性腹腔注射野百合碱 (monocrotaline ,MCT ,6 0mg·kg-1)复制PAH动物模型 ,RyR亚型的确定及其mRNA水平变化用RT PCR法测定 ,并应用Westernblot检测PAH大鼠RyR蛋白表达的变化。结果　大鼠PASMC只表达RyR 3个亚型中的Ⅱ亚型(RyR2 ) ,在PAH时RyR2的mRNA表达量 (以RyR2区带与β actin区带的密度百分比表示 )为 85 18%± 11 17% ,高于对照组 36 87%± 7 30 % (P <0 0 1) ,且RyR2蛋白水平亦升高 ,为对照组的 170 2 3 %± 45 34% ,(P <0 0 1)。结论　肺动脉平滑肌细胞存在RyR2亚型 ,其表达增强可能参与了PAH的发生。AIM To investigate the exact subtype of ryanodine receptor (RyR) in pulmonary artery smooth muscle cell(PASMC) and the change of RyR expression from pulmonary hypertensive (PAH) rats. METHODS Rats pre treated with a single intraperitoneal injection of monocrotaline (MCT, 60 mg·kg -1 ) were used to produce PAH animal model and killed 21 d later. The subtype expression of RyR and the change of their mRNA were measured by RT PCR; protein expression of RyR was measured by Western blot in PASMC. RESULTS The PASMC of rats only expressed type ⅡRyR (RyR2). RT PCR showed the RyR2 mRNA concentration of PAH group was higher than that of control; Western blot demonstrated that the RyR2 protein level in PAH group was increased to (170 23±45 34)%. CONCLUSION PASMC only expressed RyR2 subtype and the increment of RyR2 expression may contribute to the pathological mechanism of PAH formation.

关 键 词：Ryanodine受体亚型 肺动脉高压 血管平滑肌 

分 类 号：R543.2[医药卫生—心血管疾病]