阿魏酸钠对组胺引起的血管内皮细胞单层通透性增高的作用  

Influence of ferulic acid sodium on the enhance of endothelial permeability induced by histamine

作　　者：周吉燕[1] 樊懿[2] 韩志芬 吴大正[2] 胡之璧[2] 

机构地区：[1]上海中医药大学科研处,上海200032 [2]上海中医药大学中药研究所,上海200032

出　　处：《中国药理学通报》2000年第6期664-666,共3页Chinese Pharmacological Bulletin

摘　　要：目的　本实验观察阿魏酸钠对炎症介质组胺引起的血管内皮通透性增加的作用。方法　从新生牛主动脉分离获得单个内皮细胞 ,培养于微孔滤膜上直至形成致密单层。通过灌流Hanks液或含 5g·L-1白蛋白的Hanks液后 ,测定经 1× 10 -4 mol·L-1组胺溶液加 1× 10 -5mol·L-1阿魏酸钠处理的液体滤过系数 (Kf)、液体滤过流量 (Jv)和蛋白质渗透压反射系数 (σ)。结果　内皮单层经组胺处理后 ,Kf和Jv降低 ,σ升高 ;而阿魏酸钠能够抑制因组胺造成的Kf和Jv降低 ,以及σ升高。结论　组胺能够引起血管内皮单层通透性的增加 ,而阿魏酸钠能够减轻组胺造成的内皮单层通透性的增加 ;其作用机制需要进一步地研究。AIM To study influence of ferulic acid sodium on the increase of endothelial permeability induced by histamine. METHODS The endothelial cells isolated from bovine aorta were cultured on polycarborate microporous filter membrane to develop confluent endothelial monolayer. Fluid filtration coefficient (Kf ), filtration volume ( Jv ) and osmotic reflective coefficient (σ ) to protein were measured in the endothelial monolayer treated by 1×10 -4 mol·L -1 histamine for 30 min or treated by 1×10 -4 mol·L -1 histamine for 30 min preincubated 1×10 -5 mol·L -1 ferulic acid sodium for 60 min after perfused either Hanks balanced salt solution or Hanks balanced salt solution containing 5 g·L -1 albumin. RESULTS Kf, Jv were increased after perfused Hanks balanced salt solution, and Kf , Jv were increased and σ decreased after perfused Hanks balanced salt solution containing 5 g·L -1 albumin in the endothelial monolayer treated by 1×10 -4 mol·L -1 histamine. Application of ferulic acid sodium in the endothelial monolayer treated with histamine induced reducing of Kf , and Jv after perfused Hanks balanced salt solution, and induced reduce of Kf , and Jv and σ enhance after perfused Hanks balanced salt solution containing 5 g·L -1 albumin, compared with in the endothelial monolayer treated with histamine purely. CONCLUSION Increases in the endothelial monolayer permeability induced by histamine, and ferulic acid sodium can inhibit increases in the endothelial monolayer permeability induced by histamine. The cellular mechanism of permeability response induced by ferulic acid sodium remain to be further elucidated.

关 键 词：阿魏酸钠 组胺 内皮单层 通透性 血管内皮细胞 

分 类 号：R285.5[医药卫生—中药学]