肾上腺髓质素对血管紧张素II的促血管平滑肌细胞增殖作用的影响  

Effect of proadrenomedullin on proliferation of vascullar smooth muscle cells induced by Ang Ⅱ

作　　者：马健[1] 杨晔[2] 李载权[3] 唐朝枢[3] 

机构地区：[1]海军401医院内一科,青岛266071 [2]海军总医院,北京100037 [3]北京医科大学第一医院心血管基础研究所,北京100034

出　　处：《基础医学与临床》2000年第6期525-527,共3页Basic and Clinical Medicine

摘　　要：观察肾上腺髓质素前体N端20肽（PAMP）和肾上腺髓质素（ADM ）对血管紧张素II（AngII）诱导的血管平滑肌细胞（VSMC）增殖的影响。用测定培养的大鼠VSMC3H-TdR掺入和蛋白激酶C（PKC）活性的方法。结果发现：10-8mol/L AngII刺激培养的VSMCs 3H-TdR掺入增加2.68倍（P＜0.01）、PKC活性增加1.02倍（P＜0.01）。而10-9mol/L-10-7mol/L的PAMP或ADM与10-8mol/L AngII同时孵育，则显著抑制AngII的上述作用（P＜0.01）。且两者的抑制作用无明显差异。PAMP和ADM均有抑制AngII所致的VSMC增殖作用，可能是通过抑制信号传导系统中的PKC活性来实现的。PAMP和ADM 与AngII相互作用，共同调节VSMC的增殖，从而维持循环系统功能的稳态。To observe the effect of proadrenomedullin N-term inal 20 peptide (PAM P)and adrenomedullin (ADM)on AngII-stimulated proliferation of vascular smooth m uscle cells(VSMCs). The 3H-TdR incorporation and intracellular PKC activity were measured in the cultured rat VSMCs. This study demonstrates that AngII (10-8 mol/L)significantly increased the VSMCs 3H-TdR inco rporation by 2.68-fold( P ＜0.01) and PKC activity by 1.02-fold( P ＜0.0 1).PAMP and ADM ( 10-9～10-7 mol/L) markedly inhibited the AngII -s timulated 3H-TdR incorporation and PKC activation in a c oncentration-dependent manner .Conclusion: PAMP and ADM possess the ability to inhibit the AngII-induced proliferation of VSMCs through inhibiting the PKC act ivity. It suggests that PAMP,ADM and AngII may play a role to maintain the circ ulatory homeostasis by interact of each other.

关 键 词：肾上腺髓质素 血管紧张素II 血管平滑肌细胞 VSMC增殖 高血压 

分 类 号：R341[医药卫生—基础医学]