γ-氨基丁酸对离体大鼠黄体化颗粒细胞孕酮分泌的影响  

Effect of GABA on progesterone secretion in rat luteinising granulosa cells in vitro

作　　者：姜杰[1] 倪江[2] 李庆雷[2] 边淑玲[2] 张伟[2] 

机构地区：[1]解放军第211医院麻醉科,哈尔滨150080 [2]哈尔滨医科大学生理教研室,哈尔滨150086

出　　处：《基础医学与临床》2000年第6期547-548,共2页Basic and Clinical Medicine

摘　　要：用离体孵育方法，观察γ-氨基丁酸(GABA)对大鼠黄体化颗粒细胞孕酮分泌的影响，同时观察在PMSG、蝇覃醇、放线菌酮、forskolin作用下，GABA对黄体化颗粒细胞孕酮分泌的影响。结果显示GABA促进大鼠黄体化颗粒细胞孕酮分泌，蝇覃醇有同样作用。GABA减弱PMSG对颗粒细胞孕酮分泌的促进作用。GABA在放线菌酮作用下对颗粒细胞孕酮分泌无显著影响。GABA促进forskolin作用下的颗粒细胞孕酮分泌。提示GABA促进大鼠黄体化颗粒细胞孕酮分泌是通过结合到颗粒细胞上的GABA受体，可能与细胞内腺苷酸环化酶系统有关，而与蛋白质合成无关。It has been recently showed that GABA plays an important r ole in the regulation of ovarian function. The present study was to evaluate t he effect of GABA on progesterone production using rat luteinising granulosa ce lls in vitro . The lutei nising granulosa cells were cultured in KRBGAL for 1 h, the cells were treated w ith reagents for a further 2 h. The secretion of progesterone was assayed by ra d ioimmunoassay. The results showed that GABA stimulated progesterone secretion ， GABA agonist muscimol had the same effect.GABA decreased PMSG induced progester o ne secretion.Under the influence of cycloheximide, the effect of GABA on proges t erone secretion had no significant difference. With the effect of GABA and fors k olin , progesterone secretion had been increased. The results indicated that GA B A stimulated progesterone secretion by rat luteinising granulosa cells in vitr o and its effect was mediated by GABA receptor and adenylate cyclase-cAMP mess enger system.

关 键 词：Γ-氨基丁酸 黄体化颗粒细胞 孕酮 

分 类 号：R341[医药卫生—基础医学]