低温冷冻对脐血CD_(34)^+细胞和造血祖细胞增殖的影响  

Effect of Hypothermic Freezing on the Proliferation of CD_(34)^+ Cells and Hematopoietic Progenitors in Umbilical Cord Blood

作　　者：胡丽华[1] 余忠清[1] 曹奎杰[1] 殷开华 张青[1] 

机构地区：[1]同济医科大学附属协和医院输血科,武汉430022

出　　处：《同济医科大学学报》2000年第6期567-568,580,共3页Acta Universitatis Medicinae Tongji

摘　　要：采用－８０℃低温冷冻法，观察冷冻前及冷冻后１、２、３周时单个核细胞（ＭＮＣ）的回收率，脐血造血前体细胞在体外的增殖力，并用流式细胞仪检测ＣＤ３４＋细胞的百分率。旨在探讨低温冷冻对脐血造血前体细胞的影响。结果表明：脐血冷冻后１、２、３周时ＭＮＣ的回收率分别为９３．６％，９１．５％和９０．７％（Ｐ＞０．０５）；细胞活力分别为９８％，９７％，９５％和９１％（Ｐ＞０．０５）；ＣＤ３４＋细胞依次为１．２０％、１．１８％、１．１６％和１．１７％；ＣＦＵ－ＧＭ，ＣＦＵ－Ｅ冷冻后１、２周时回收率达９７％和９５％（Ｐ＞０．０５），第３周时达８９％（Ｐ＞０．０５）。结果提示：长期冷冻可能导致冷冻之脐血祖细胞产率下降，但对代表造血干细胞的ＣＤ３４＋细胞影响则不大。To explore the effect of hypothermic freezing on hematopoietic progenitors in umbilical cord blood, -80℃ hypothermic freezing method was used to observe the recovery of mononuclear cells (MNC), ex- amine the proliferative activity in vitro of progenitors in umbilical cord blood and analyzed the positive CD34+ cells by flow cytometer before freezing and 1, 2, 3 weeks after freezing. The results showed that the recovery of MNCs in umbilical cord blood 1, 2, 3 weeks after freezing was 93.6%, 91.5% and 90.7%, respectively (P>0.05). The cell proliferative activity was 98%, 97%, 95% and 91% (P>0.05) and the percentage of CDL cells 1.20 %, 1.l8 %, 1.16% and 1.17% before freezing and 1, 2, 3 weeks after freezing, respec- tively. The recovery of CFU-GM or CFU-E at the third week after cyropreservation was 89% (P>0.05). It was suggested that hypothermic freezing for a long time might result in a decrease in the production of umbilical cord blood progenitors, but had a little influence on CD34+ cells.

关 键 词：脐血 CD34^+细胞 低温冷冻 造血祖细胞 细胞增殖 

分 类 号：R454.5[医药卫生—治疗学] R331.14[医药卫生—临床医学]