A型肉毒毒素(BoNT/A)表位多肽单克隆抗体的制备及其效价评定  

Preparation and titer evaluation of monoclonal antibody against botulinum neurotoxin serotype A( BoNT /A)

作　　者：韦耀[1,2] 韩朋[1] 周旋[1] 孙琦[1] 张宝[1] 万成松[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院BSL-3实验室,广东广州510515 [2]西双版纳傣族自治州疾病预防控制中心性艾防治科,云南景洪666100

出　　处：《中国卫生检验杂志》2013年第18期3510-3513,共4页Chinese Journal of Health Laboratory Technology

基　　金：广东省社会发展领域科技计划项目(2010B031000005)

摘　　要：目的获得高效价A型肉毒毒素（BoNT／A）表位多肽单克隆抗体。方法利用DNASTAR分析软件，结合Expasy网络服务器，综合参考多参数预测B细胞抗原表位；以多肽作为抗原制备BoNT／AHc表位多肽单克隆抗体，并进行效价鉴定。结果以合成多肽（P1＆P2）分别免疫Balb／c小鼠，获得两株单克隆抗体，经鉴定分别是IgG和IgM，效价为10-5～10-6；检测抗体结果显示，纯度较高，特异性好。结论本研究制备出了特异性好、效价较高的A型肉毒毒素单克隆抗体，为获得抗A型肉毒毒素的快速现场检测提供技术支持。Objective To obtain high - titer monoclonal antibody to botulinum toxin type A （BoNT/A）. Methods DNAS- TAR analysis software and Expasy network server were combined to predict the B cell antigenic epitopes, refering to multiple pa- rameters. BoNT/A Hc epitope polypeptide monoclonal antibody was produced based on designed polypeptide, then the titer was evaluated. Results BALB/c mice were immunized by two synthetic peptides （P1 ＆P2） to obtain two strains of monoclonal an- tibodies （ IgG and IgM）, pure and specific, with the titers of 10 -5 - 10-6. Conclusion The high - specificity and high - titer monoclonal antibodies against botulinum toxin A were prepared, thus providing a technical support for the rapid field detection of botulinum toxin type A.

关 键 词：A型肉毒毒素 单克隆抗体 效价 

分 类 号：S852.43[农业科学—基础兽医学]