川穹嗪联合胚胎干细胞治疗大鼠肺纤维化的机制研究  

Research on the therapeutic effect of Tetramethylpyrazine and Embryonic stem cells in pulmonary fibrosis of rats

作　　者：刘延梅[1] 马少君[1] 苗青[1] 尙亮 

机构地区：[1]陕西省人民医院西院二病区,西安710068

出　　处：《陕西医学杂志》2014年第1期10-14,共5页Shaanxi Medical Journal

基　　金：陕西省科学技术研究发展计划项目(No 2012k16-01-01)

摘　　要：目的:研究静脉注射胚胎干细胞(ESC)和川穹嗪(TMP)对肺纤维化大鼠的治疗作用。方法:气管内滴注博莱霉素8.5mg/kg制作雌性大鼠的肺纤维化模型。治疗组分别单独(联合)静脉注射大鼠ESC与川穹嗪,对照组注射生理盐水。记录大鼠的生存状态,测定大鼠肺组织的羟脯氨酸含量,肺脏病理学观察炎症状态、细胞凋亡情况以及相关蛋白的变化。结果:与对照组相比,接受ESC与TMP联合治疗后,肺纤维化模型大鼠的体质量增加,状态良好;肺羟脯氨酸含量明显降低;肺脏病理检查显示肺组织炎症程度降低,结构破坏减轻。流式细胞结果显示细胞凋亡指数降低,其核心蛋白MMP-2和MMP-9的表达明显下调。结论:静脉注射ESC与TMP可以减轻博莱霉素诱导的大鼠肺部炎症和肺纤维化。Objective： To investigate the therapeutic effect of tetramethylpyrazine and embryonic stem cells in pulmonary fibrosis of rats. Methods： IntratracheaI instillation of the bleomycin 8.5 mg/kg to produce female rats model of pulmonary fibrosis. Treatment groups were injected with ESC or/and Chuanqiongqin, compared with the control group with saline. The survival state of the rats were recorded. Meanwhile, the content of hypdroxypro- line （Hyp） in lung tissue was assayed. Detection of the lung pathology, cell apoptosis and the activities of apoptosis related proteins were processed. Results： Compared with the control group, the group of ESC and TMP therapy had a good condition with the increasing of body weight; lung hydroxyproline content was significantly reduced; lung pathological examination showed a lower degree of lung tissue inflammation, with the mitigation of structural dam age. The apoptotic index was decreased by flow cytometry, and the expression of MMP-2 and MMP-9 were signifi cantly down regulated. Conclusion：ESC and TMP might be a therapeutic medicine for pulmonary fibrosis.

关 键 词：肺纤维化 药物作用 肺纤维化 病理生理学 干细胞 治疗应用 伞形科 治疗应用动物 实验 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统]