水飞蓟素对UVB致人角质形成细胞光老化保护作用的研究  

Protective Effects of UVB Induced Photoaging in HaCaT by Silymarin

作　　者：石玉生[1] 张艳[1] 马金龙[1] 彭玉博 宋丽艳[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《中医药信息》2013年第6期36-39,共4页Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究面上项目(12511533)

摘　　要：目的:对水飞蓟素中的主要成分水飞蓟宾、异水飞蓟宾、水飞蓟宁、水飞蓟亭的抗细胞光老化作用进行研究。方法:采用UVB辐射HaCaT进行造模并设置空白对照组,运用酶生化法检测水飞蓟宾、异水飞蓟宾、水飞蓟宁、水飞蓟亭对UVB辐射HaCaT后的细胞增殖及抗氧化酶的影响,确定水飞蓟素中抗HaCaT细胞光老化的主要有效成分。结果:与模型组相比,水飞蓟素中的水飞蓟宾、异水飞蓟宾、水飞蓟宁、水飞蓟亭均可增强细胞的增殖活力、提高细胞中SOD的活性、降低MDA的产生和LDH的漏出量。结论:水飞蓟素中的水飞蓟宾、异水飞蓟宾、水飞蓟宁、水飞蓟亭对UVB致人角质形成细胞光老化均具有保护作用,其中水飞蓟宁和水飞蓟亭的抗光老化作用更为显著。Objective：To research the effects of anti - cell photoaging about the main components of Silymarin such as silibinin, iso silibinin , silymarin Ning, Silychristin. Methods ： Use HaCaT by UVB radiation as model. Detect the affection of silibinin, iso silibinin, silymarin Ning, Silychristin in cell multiplication and antioxi- dase by using Enzyme biochemical method. Make sure the main effective constituents which could induce anti -photoaging HaCaT cells in Silymarin. Results：Compared with the model group, silibinin, iso silibinin, silymarin Ning, Silychristinc could increase cell proliferation activity, increase SOD activity in cell, reduce the production of MDA and LDH. Conclusion ： Silibinin, iso silibinin, silymarin Ning, Silychristinc can protect UVB induced photoaging in HaCaT. The effects of Silvmarin Nine. Silvc, hri^tlno ~r ;,,,,,;~,~

关 键 词：水飞蓟素 光老化 人角质形成细胞 

分 类 号：R285.5[医药卫生—中药学]