解淀粉芽孢杆菌BGP20-γ的诱变选育及生防效果  被引量:4

Mutation Breeding of Bacillus amyloliquefaciens BGP20-γ and Its Biocontrol Effect

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作  者:赵延存[1] 李鹏霞[1] 黄开红[1] 王毓宁[1] 孙娅[1] 胡花丽[1] 

机构地区:[1]江苏省农业科学院农产品加工研究所,江苏南京210014

出  处:《核农学报》2013年第12期1791-1796,共6页Journal of Nuclear Agricultural Sciences

基  金:江苏省博士后基金(2011年第二批);江苏省农业科学院博士后基金(6511106);江苏省农业科技自主创新资金[CX(12)1005-07]

摘  要:胡萝卜软腐欧文氏菌(Erwinia carotovora subsp.carotovora,Ecc)引起的细菌性软腐病是导致采后蔬菜腐败的主要原因之一,前期研究发现解淀粉芽孢杆菌(Bacillus amyloliquefaciens)BGP20对该病害具有较好的生防效果。为了进一步提高BGP20对采后蔬菜细菌性软腐病的生防效果,本研究利用60Co辐照诱变技术,对该菌株进行诱变选育。结果表明,在200-800 Gy剂量范围内,60Co对BGP20的致死率随着辐照剂量的增加而迅速上升。600 Gy剂量的60Co辐照致死率为78.3%,选用该剂量进行诱变选育。利用辣椒对409株突变菌株的生防效果进行活体评价,获得1株生防效果显著提高的突变菌株BGP20-γ(P<0.05)。与野生型菌株BGP20相比,利用突变株BGP20-γ处理的辣椒伤口腐烂面积下降64.4%,其在辣椒伤口上的定殖能力显著提高,将病原菌Ecc的群体数量限制在一个更低的水平。Bacillus amyloliquefaciens BGP20 was a promising candidate in biological control of postharvest bacterial soft rot of vegetables caused by Erwinia carotovora subsp, carotovora (Ecc). In order to further improve its biocontrol effect against bacterial soft rot, the mutation breeding of B. amyloliquefaciens BGP20 was carried out by 6~Co irradiation. This study showed that the mortality rate of BGP20 increased with increasing radiation dose from 200 Gy to 800 Gy. The 600 Gy radiation dose was used in the mutation breeding in which the mortality rate was 78.3%. The biocontrol effects of 409 mutant strains were evaluated in peppers. Among them, a mutant strain called BGP20-~/ had a higher biocontrol effect compared to the wild strain BGP20. The lesion area of peppers treated by BGP20-~/ was decreased by 64.4% compared to that of peppers treated by BGP20. In addition, the mutant strain BGP20-Y had stronger colonizing ability in peppers than the wild strain BGP20, and the viable count of the pathogenic bacteria Ecc was suppressed to a low level.

关 键 词:解淀粉芽孢杆菌 胡萝卜软腐欧文氏菌 60Co 诱变育种 群体动态 

分 类 号:S476[农业科学—农业昆虫与害虫防治]

 

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