Gene Expression Profiling Related to Hyphal Growth in a Temperature-Sensitive Mutant of Magnaporthe oryzae  

作　　者：LI Xue-song XU Fei WANG Hong-kai LIN Fu-cheng 

机构地区：[1]State Key Laboratory for Rice Biology,Biotechnology Institute,Zhejiang University [2]Bioinformatics Lab,Institute of Digital Agriculture,Zhejiang Academy of Agricultural Sciences

出　　处：《Journal of Integrative Agriculture》2013年第12期2189-2196,共8页农业科学学报（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang Province,China(Y3110028 and LQ12C14003)

摘　　要：The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food security.Hyphal growth is the foundation of fungal development and proliferation of fungi.To investigate genes involved in hyphal growth of this fungus,digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity,with its related wildtype strain.416 genes were detected as differential expression,178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant.Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuole- related processes.The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M.oryzae pathogenesis.The rice blast,caused by fungus Magnaporthe oryzae,is a major constraint to the world food security.Hyphal growth is the foundation of fungal development and proliferation of fungi.To investigate genes involved in hyphal growth of this fungus,digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity,with its related wildtype strain.416 genes were detected as differential expression,178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant.Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuole- related processes.The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M.oryzae pathogenesis.

关 键 词：differential expressed gene QRT-PCR gene ontology hyphal growth VACUOLE 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]