Effect of Swim-Up and Percoll Treatment on Sperm Quality and In vitro Embryo Development in Yak  

作　　者：LIU Ben CUI Yan YU Si-jiu 

机构地区：[1]Academic of Veterinary Medicine,Gansu Agricultural University [2]Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat

出　　处：《Journal of Integrative Agriculture》2013年第12期2235-2242,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(30871903);the Fundamental Research Funds for the Gansu Universities,China(2011)

摘　　要：This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development.Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods.Sperm concentration,progressive motility,recovery of motile sperm,membrane integrity,acrosome and chromatin integrity were scored and compared in recovered samples and controls.In addition,the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated.Swim-up separated sperm showed a higher motility,while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation.According to the optical and electron microscopies,swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation.However,there was no difference in the percentage of sperm with intact chromatin between two treatment groups.Cell numbers in the blastocysts of two groups were not different.The blastocyst rate was similar in both groups,whereas cleavage rate was higher when swim-up was used.This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development.Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods.Sperm concentration,progressive motility,recovery of motile sperm,membrane integrity,acrosome and chromatin integrity were scored and compared in recovered samples and controls.In addition,the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated.Swim-up separated sperm showed a higher motility,while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation.According to the optical and electron microscopies,swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation.However,there was no difference in the percentage of sperm with intact chromatin between two treatment groups.Cell numbers in the blastocysts of two groups were not different.The blastocyst rate was similar in both groups,whereas cleavage rate was higher when swim-up was used.

关 键 词：YAK SWIM-UP percoll gradient centrifugation embryo development 

分 类 号：S823.85[农业科学—畜牧学]