基于量效融合评价的正交优选核桃楸皮抗肝SMMC-7721肿瘤黄酮类成分的提取工艺  

Optimized Extraction Technology of Flavonoid Compounds with Anti-SMMC-7721 Tumor Activities in Bark of Juglans mandshurica by Orthogonal Experimental Design based on Dose-Effect Fusion Evaluation Method

作　　者：郑莹[1] 王帅[1] 孟宪生[1] 包永睿[1] 

机构地区：[1]辽宁中医药大学药学院/辽宁省组分中药工程技术研究中心,辽宁大连116600

出　　处：《中药材》2013年第10期1686-1689,共4页Journal of Chinese Medicinal Materials

基　　金：国家"十二五""重大新药创制"专项(2013ZX09507005-004)

摘　　要：目的:优选核桃楸皮药材中抗肿瘤有效组分黄酮类成分的最佳提取工艺,并探究黄酮类成分含量与药效学指标间的相关性。方法:以核桃楸皮抗肿瘤有效组分的提取率、出膏率及MTT法检测细胞抑制率为指标综合评分,采用正交试验设计优化有效组分提取的主要影响因素,并通过SPSS 17.0软件对有效组分与药效学指标进行Pearson相关系数分析。结果:核桃楸皮抗肿瘤有效组分总黄酮的最佳提取工艺为:70℃下,以20倍量60%乙醇提取3次,每次2 h,且总黄酮提取率与体外细胞抑制率呈正向直线相关。结论:用提取工艺参数与药效双重指标相结合的方法优选核桃楸皮总黄酮最佳提取工艺,为中药提取工艺的优化提供了一种新的思路,同时也为核桃楸皮临床应用提供了可靠的实验依据。Objective ：To optimize the extraction technology of total flavonoids with antineoplastic activities in Juglans mandshuri- ca, and explore the correlation between total flavonoids and pharmacodynamics indicators. Methods： The quantity of antineoplastic components, ratio of extraction and cell inhibition rate were taken as the comprehensive indexes to optimize the main factors that influ- ence the extraction of effective components by orthogonal experiment design. SPSS 17.0 software was used to analyze the Pearson corre- lation between effective components and phannacodynamics indexes. Results： The best extracting condition of total flavonoids were as follows： the ratio of 60% ethanol to Juglans mandshurica was 20：1 ,extracting for 3 times, each time for 2 hour at 70~C. Flavonoids extraction yield and cell inhibition rate was positively related in the straight line. Conclusion： This study provides a new insight into the optimization of extraction technology for traditional Chinese medicine, and lays a safe and reliable experimental basis for the clinical application of Juglans mandshurica.

关 键 词：核桃楸皮 总黄酮 正交试验设计 药效学指标 

分 类 号：R286[医药卫生—中药学]