聚合酶链反应技术检测旋毛虫DNA的实验研究  

EXPERIMENT RESEARCH ON DETECTING TRICHINELLA SPIRALIS BY PCR

作　　者：温艳[1] 张月清[1] 

机构地区：[1]北京热带医学研究所,北京100050

出　　处：《中国人兽共患病杂志》2001年第1期61-62,共2页Chinese Journal of Zoonoses

摘　　要：目　的　建立敏感、特异、稳定的聚合酶链反应 (PCR)法 ,以检测旋毛虫病。方法　采用PCR法 ,选择与合成引物并确立最佳扩增条件 ,比较不同的提取DNA方法 ,检测血浆中旋毛虫幼虫。结果　经扩增于 5 0 0bp、6 70bp、12 0 0bp出现特异性片段 ,与原选择的编码基因片段大小相同 ,此特异片段与溶组织内阿米巴、日本血吸虫DNA无交叉反应 ,不同的DNA提取法结果显示 :用裂解煮沸法同样可从仅含 1条旋毛虫幼虫的 2 5 μl正常小鼠血浆中扩增出特异性片段。 结论　PCR法检测旋毛虫敏感高、特异强 。Aim To establish a sensitive specific and stable polymerase chain reaction (PCR) for detecting Trichnilla spiralis infection. Method To adopt the PCR technique、to make sure the best choice of amplification and to compare the drawing DNA methods of lysine boiling with phenol extraction. Results A single excysted larva in plasma was identified. Due to the different homologies of the A1 、B1 of the 1.6-kb fragment, 3 bands of 500bp、670bp and 1200bp usually were present. It was not cross- reaction with Entamoeba histolytica and Schistosoma japonica.It is a simply and better method to extract the DNA with lysine boiling. Conclusion It shows that this technique can be used in early clinical diagnosis and animal infection. PCR technique can replace biopsy of the muscle tissue..

关 键 词：旋毛虫 DNA 聚合酶链反应 PCR 

分 类 号：R532.140.4[医药卫生—内科学]