脐带造血干细胞和巨核细胞microRNA表达谱的差异  

Microarray Analysis of Differential Expressed MicroRNAs Between Human Umbilical Cord Hematopoietic Stem Cells and Megakaryocytes

作　　者：姚根宏[1] 张国栋[2] 吴永政[1] 

机构地区：[1]南京军区南京总医院输血科,江苏南京210002 [2]南京医科大学,江苏南京210029

出　　处：《中国血液流变学杂志》2013年第3期413-415,共3页Chinese Journal of Hemorheology

基　　金：南京军区医学科技创新重点项目（112026）,南京军区南京总医院科研基金（2011048,2011050）

摘　　要：目的探讨定向诱导造血干细胞分化为巨核细胞和血小板的分子机制。方法将脐带造血干细胞培养后，分离巨核细胞，分别提取造血干细胞和巨核细胞总RNA，进行microRNA芯片分析。结果与造血干细胞相比，巨核细胞中表达上调超过2倍的microRNA有183个，下调超过2倍的microRNA有139个。结论造血干细胞和巨核细胞microRNA表达谱存在差异，尤其是多个microRNA与定向分化巨核细胞相关。Abstract： Objective To elucidate the mechanism of megakaryocyte development and platelet production from hematopoietic stem cells. Methods CD34＋ cells were isolated from umbilical cord blood and cultivated. Megakaryocytes were purified from cultivated CD34＋ cells.Total RNAs from CD34＋ cells and megakaryocytes were extracted.Microarray analysis was performed to determine differential expressed microRNAs.Results Differential microRNAs were defined as a statistically significant difference with 2 fold or greater change. Compared with hematopoietic stem cells,183 microRNAs showed higher expression and 139 microRNAs were down-regulated in megakaryocytes.Conclnsion The differential expressed microRNAs between hematopoietic stem cells and megakaryocytes indicates that some microRNAs play an important role in regulating megakaryocytes differentiation from hematopoietic stem cells.

关 键 词：脐带造血干细胞 巨核细胞 MICRORNA 

分 类 号：R457.12[医药卫生—治疗学]