乙腈盐析萃取HPLC-PDA同时测定水中11种苯胺类化合物  

Simultaneous Determination of Eleven Anilines in Water by Acetonitrile Salt-out Extraction HPLC-PDA

作　　者：母应锋[1] 王丽媛[1] 胡恩宇[1] 孙娟[1] 宋祖华[1] 

机构地区：[1]南京市环境监测中心站,江苏南京210013

出　　处：《中国环境监测》2013年第6期102-107,共6页Environmental Monitoring in China

摘　　要：通过对色谱分析和样品萃取条件的选择和优化，建立了同时分析水中11种苯胺类化合物的HPLC方法。样品经乙腈盐析萃取后直接进样分析，采用ODS色谱柱，以乙腈-水为流动相进行梯度洗脱，用PDA检测。结果表明，11种苯胺类化合物在0．20—100mg／L范围内其浓度和检测信号呈良好的线性关系，方法检出限为0．002～0．007mg／L，地表水和废水样品加标回收率为81．6％～97．4％，相对标准偏差为1．5％～5．5％。A new analytical method for the simultaneous determination of eleven phenyl amines compounds in water sample using high liquid chromatography has been developed. Parameters, such as chromatographic conditions and extraction conditions were controlled and optimized. After salting-out extraction with acetonitrile,the extraction solution was analyzed by HPLC-PDA using ODS column with gradient elution using acetonitrile-water as mobile phase. The developed protocol was found to yield good linear calibration curves in a concentration range from 0. 20 to 100 mg/L for eleven phenyl amines compounds. The limits of detection were found to be in the range between 0. 03 and 0.08 mg/L. The recoveries from surface water and waste water fortified samples were 81.6% -97.4% ,respectively： The relative standard deviations of the method were 1.5% - 5.5%.

关 键 词：高效液相色谱法 二极管阵列检测器 盐析萃取 苯胺类化合物 水 

分 类 号：X830.2[环境科学与工程—环境工程]