丹参联合β-榄香烯对肝星形细胞LX-2增殖和凋亡的影响  

Effect of β-Elemene Combined with Radix Salviae Miltiorrhizae on the Proliferation and Apoptosis of LX-2 Cell

作　　者：马力天[1,2] 任秦有[1] 白杨[2] 李成花 杨建栋 孙世友[2] 周睿[2] 杜乐[5] 郑瑾[1,6] 

机构地区：[1]第四军医大学唐都医院中医科暨中西医结合肿瘤科 [2]第四军医大学学员旅 [3]陕西省中医医院 [4]辽宁省海城市65547部队医院 [5]第四军医大学放射医学教研室 [6]解放军总医院中医院

出　　处：《现代生物医学进展》2013年第34期6626-6629,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81072973);省级中医药临床研究课题(lc70)

摘　　要：目的:探讨丹参联合β-榄香烯对肝星形细胞LX-2增殖和凋亡的影响。方法:体外培养肝星形细胞LX-2,分别将丹参(浓度为1、2、4、6、8 mg/ml),β-榄香烯(浓度为25、50、100、150、200μg/ml),单独作用于LX-2细胞后24 h、48 h用CCK-8(Cell Count Kit-8)法检测细胞的增殖情况,并选取合适的药物浓度(丹参3.6 mg/ml,β-榄香烯125μg/ml),然后进行联合用药,加药24 h、48 h后用CCK-8(Cell Count Kit-8)法检测细胞的增殖情况,用流式细胞术检测细胞的凋亡率。结果:①CCK-8法显示丹参(浓度为1、2、4、6、8 mg/ml),β-榄香烯(浓度为25、50、100、150、200μg/ml)作用LX-2细胞24 h、48 h后,其增殖抑制率均明显高于正常对照组(P<0.05),并且呈浓度和时间依赖性。②联合用药(丹参3.6 mg/ml,β-榄香烯125μg/ml)时,LX-2细胞的增殖抑制率和凋亡率均显著高于单独用药(P<0.01)。结论:丹参、β-榄香烯单独或二者联合作用均能抑制LX-2细胞的增殖,且联合应用的作用显著高于单独用药,可协同促进LX-2细胞的凋亡。Objective： To investigate the effect of β-Elemene combined with Radix Salviae Miltiorrhizae on the proliferation and apoptosis of LX-2 cell. Methods： Different concentrations ofl3-Elemene injection （25, 50, 100, 150, 200 i^g/ml） and Radix Salviae Miltiorrhizae（1, 2, 4, 6, 8 mg/ml） were solo applied to LX-2 cells. Cell proliferation was measured by CCK-8（Cell Count Kit-8）. The appropriate drug concentration（3.6 mg/ml Radix Salviae Miltiorrhizae, 125 μg/ml β-Elemene） were selected. Then, β-Elemene combined with Radix Salviae Miltiorrhizae injection was applied to LX-2 cells. Cell proliferation was measured by CCK-8 （Cell Count Kit-8）, cell apoptosis was detected by flow cytometry. Results： （L）Cell proliferation were dramatically inhibited by β-Elemene injection （25, 50, 100, 150, 200 μg/ml） and Radix Salviae Miltiorrhizae（1, 2, 4, 6, 8 mg/ml） at 24 h, 48 h （P〈0.05）. In addition, Cell proliferation were dramatically inhib- ited by injection β-Elemene combined with Radix Salviae Miltiorrhizae. ②Combination therapy, apoptosis rate and inhibition rate of proliferation in LX-2 cells treated by β-Elemene combined with Radix Salviae Miltiorrhizae were higher than the monotherapy. Conclusion： Salvia miltiorrhiza, β-Elemene and Salvia miltiorrhiza combined with elemene can inhibit the proliferation, accelerate the apoptosis in LX-2 cells. What＇s more, Salvia miltiorrhiza combined with β-Elemene have synergistic effect on the apoptosis in LX-2 cells,

关 键 词：榄香烯 丹参 LX-2细胞 增殖 凋亡 

分 类 号：R34[医药卫生—基础医学]