RP-HPLC测定龙胆泻肝口服液中黄芩甙含量  

Determination of Baicaline in Longdanxiegan Oral Liquid by RP-HPLC

作　　者：莫可元[1] 莫志江[1] 

机构地区：[1]广西壮族自治区人民医院,广西南宁530021

出　　处：《中成药》2000年第11期764-765,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :建立RP HPLC测定龙胆泻肝口服液中黄芩甙含量的方法。方法 :固定相为Nova PakC18反相柱 ;流动相为甲醇 水 磷酸 (4 5∶5 5∶0 .2 ) ;检测波长为 2 76nm。结果 :该方法的线性范围为 0 .18～ 1.79μg(r =0 .9999,n =6 ) ,平均回收率为 10 1.94% ,RSD =3 .11% (n =5 )。结论 :本法简便 ,准确 ,灵敏度高 ,重复性好 ,可用于该制剂的成分含量测定。Objective: To establish a method of RP HPLC for determination of baicaline in Longdanxiegan Oral Liquid.Methods: RP HPLC analysis was carried out on Nava Pak C 18 column and in methanol water phosphoric acid (45∶55∶0.2) as the mobile phase. The detection wavelength was 276nm. Results: The method was linear at the range of 0.18～1.79μg (r=0.9999, n=6), and the average recovery was 101.94%, RSD was 3.11% (n=5). Conclusion: The method is simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of baicaline in Longdanxiegan Oral Liquid.

关 键 词：龙胆泻肝口服液 黄芩甙 反相高效液相色谱法 含量 

分 类 号：R286.0[医药卫生—中药学]