Development of SSR Markers for a Phytopathogenic Fungus,Blumeria graminis f.sp.tritici,Using a FIASCO Protocol  

作　　者：WANG Meng XUE Fei YANG Peng DUAN Xia-yu ZHOU Yi-lin SHEN Chong-yao ZHANG Guo-zhen WANG Bao-tong 

机构地区：[1]Institute of Plant Protection,Chinese Academy of Agricultural Sciences [2]Department of Plant Pathology,China Agricultural University/Key Laboratory of Plant Pathology,Ministry of Agriculture [3]Huai'an Entry-Exit Inspection and Quarantine Bureau [4]College of Agronomy,Shihezi University [5]College of Plant Protection,Northwest A&F University

出　　处：《Journal of Integrative Agriculture》2014年第1期100-104,共5页农业科学学报（英文版）

基　　金：supported by the National Basic Research Program of China(2006CB100203 and 2011CB100403);the Key Technologies R&D Program of China during the 11th Five-Year Plan period(2006BAD08A05);the Special Fund for Agro-Scientific Research in the Public Interest(3-15),China

摘　　要：Simple sequence repeats （SSR） have been widely used as molecular markers due to their abundance and high polymorphism, However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis f.sp. tritici. From （AC）10 and （AG）10 enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO （fast isolation by AFLP of sequences containing repeats） protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces （cities） in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 （mean 0.025） and expected heterozygosity ranged from 0.297-0.816 （mean 0.538）. These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping ofBgt. SSR markers of Bgt need to be further explored.Simple sequence repeats （SSR） have been widely used as molecular markers due to their abundance and high polymorphism, However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis f.sp. tritici. From （AC）10 and （AG）10 enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO （fast isolation by AFLP of sequences containing repeats） protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces （cities） in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 （mean 0.025） and expected heterozygosity ranged from 0.297-0.816 （mean 0.538）. These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping ofBgt. SSR markers of Bgt need to be further explored.

关 键 词：Blumeria graminis f.sp. tritici MICROSATELLITE SSR FIASCO 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]