Benzene containing polyhydroxyalkanoates homo-and copolymers synthesized by genome edited Pseudomonas entomophila  

作　　者：SHEN Rui CAI LongWei MENG DeChuan WU LinPing GUO Kai DONG GuoXing LIU Lei CHEN JinChun WU Qiong CHEN GuoQiang 

机构地区：[1]MOE Key Lab of Bioinformatics, Department of Biological Science and Biotechnology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University [2]Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen 2100,Denmark [3]Nanjing University of Technology, College of Biotechnology & Pharmaceutical Engineering [4]Department of Chemistry, Tsinghua-Peking Center for Life Sciences, Tsinghua University [5]Center for Nano and Micro Mechanics, Tsinghua University

出　　处：《Science China(Life Sciences)》2014年第1期4-10,共7页中国科学（生命科学英文版）

基　　金：supported by the National High Technology Research and Development Program of China(2012AA023102 to Liu Lei,Guo Kai and Wu Qiong);the National Basic Research Program of China(2012CB725201 to Chen GuoQiang and Chen JinChun,2012CB725204 to Guo Kai and Wu Qiong);National Natural Science Foundation of China(31270146 to Chen GuoQiang)

摘　　要：Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology. For the first time, it became possible to synthesize controllable composition of poly（3-hydroxyalkanoate） （P3HA） consisting of 3-hydroxydodec- anoate （3HDD） and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken its t-oxidation. Cultured in the presence of 5-phenylvaleric acid （PVA）, the edited P. entomophila produced only homopolymer poly（3-hydroxy-5-phenylvalerate） or P（3HPhV）. While copolyesters P（3HPhV-co-3HDD） of 3-hydroxy-5-phenylvalerate （3HPhV） and 3-hydroxydodecanoate （3HDD） were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid （DDA）. Compositions of 3HPhV in P（3HPhV-co-3HDD） were controllable ranging from 3% to 32% depending on DDDA/PVA ratios. Nuclear magnetic resonance （NMR） spectra clearly indicated that the polymers were homopolymer of P（3HPhV） and random copolymers of 3HPhV and 3HDD. Their mechanical and thermal properties varied dramatically de- pending on the monomer ratios. Our results demonstrated the possibility to produce tailor-made, novel functional PHA using the chromosome edited P. entomophila.Microbial synthesis of functional polymers has become increasingly important for industrial biotechnology.For the first time,it became possible to synthesize controllable composition of poly(3-hydroxyalkanoate)(P3HA)consisting of 3-hydroxydodecanoate(3HDD)and phenyl group on the side-chain when chromosome of Pseudomonas entomophila was edited to weaken itsβ-oxidation.Cultured in the presence of 5-phenylvaleric acid(PVA),the edited P.entomophila produced only homopolymer poly(3-hydroxy-5-phenylvalerate)or P(3HPhV).While copolyesters P(3HPhV-co-3HDD)of 3-hydroxy-5-phenylvalerate(3HPhV)and 3-hydroxydodecanoate(3HDD)were synthesized when the strain was grown on mixtures of PVA and dodecanoic acid(DDA).Compositions of 3HPhV in P(3HPhV-co-3HDD)were controllable ranging from 3%to 32%depending on DDDA/PVA ratios.Nuclear magnetic resonance(NMR)spectra clearly indicated that the polymers were homopolymer of P(3HPhV)and random copolymers of 3HPhV and 3HDD.Their mechanical and thermal properties varied dramatically depending on the monomer ratios.Our results demonstrated the possibility to produce tailor-made,novel functional PHA using the chromosome edited P.entomophila.

关 键 词：POLYHYDROXYALKANOATES PHB Pseudomonas entomophila β-oxidation synthetic biology 

分 类 号：Q933[生物学—微生物学]