非血管化结构性骨移植对骨缺损靶区域修复的实验研究  

Experimental study of the reconditioning of non-vascularized structural bone graft in the target region of bone defects.

作　　者：葛建华[1] 薛兆龙 张忠杰[1] 鲁晓波[1] 罗雷茗[1] 

机构地区：[1]泸州医学院附属医院骨与关节外科,四川泸州646000 [2]南翔医院骨科,上海嘉定201802

出　　处：《四川医学》2013年第11期1636-1638,共3页Sichuan Medical Journal

基　　金：泸医附院重点课题(泸医院科【2009】39号)

摘　　要：目的 目的 研究不同方式的非血管化结构性骨移植治疗骨缺损的疗效.方法 用38只新西南大白兔建立骨缺损模型,随机分为A、B、C三组.A组将髂骨块与股骨应力骨小梁方向一致植于股骨缺损靶区域;B组垂直方向植骨;C组随机植骨.术后观察动物的一般情况,测定X-ray、新生毛细血管、新生骨生成量等指标.结果 股骨骨缺损缺损范围：13~14mm.三组骨缺损靶区域骨痂生长及X-ray：术后2周有少许程度区别,4周时差别明显,8周无明显差别.CD34染色阳性细胞数及新生骨量：术后2、4周AB、BC两组差别明显（P〈0.05）,8周三组无明显差别（P＞0.05）.结论 不同方式的非血管化结构性骨移植治疗骨缺损,影响骨缺损的早期愈合,移植骨沿骨小梁方向与成骨方向一致植入骨缺损靶区域,在骨愈合早期要优于另两种植骨方式.Objective To study the efficacy of the non-vascularized structural bone graft in the treatment of bone defects. Methods This study first established bone defect model, choose 38 New Zealand white rabbits, and succeed to establish femur bone defect model, which were randomly divided into A, B, C and three groups （n = 12）. take on the same side for autologous ili- ac bone graft source, and the iliac cut size equal to 8 along the trabecular bone or stress direction. Redueted the femur, and fixed by mini-plate and screw. Group A plant iliac in femoral defect with femur stress （bone trabeculae） in the same direction; Group B planting iliac block in the direction that perpendicular to the explants of femoral stress （ trabecular bone） ; Group C randomly im- planted in the femoral defect area. Observed the generral conditions of rabbits, and evaluated the X-ray and new blood capillary and positive cells of CD34 dyeing. Results The femur bone defect range ： 13 ~ 1gram. The bony callus of target area and X-ray in three groups after operation： little difference on second week, significant difference on fourth week and indifference on eighth weeks, positive cells of CD34 dyeing and new blood capillary after operation： significant difference on second or fourth week（P 〈 0. 05）, and indifference on eighth week （P 〉 0. 05）. Conclusion The non-vascularized structural bone graft in the treatment of bone defects, with the derection of trabecular bone or stress direction grafting is influential in early healing of bone defects, the graft trabecular direction and the bone in the same direction planting in the early bone healing is superior to the vertical direction of the bone grafting.

关 键 词：骨缺损 骨移植 骨小梁 再血管化 

分 类 号：R687.3[医药卫生—骨科学]