北美温带玉米杂交种选系的遗传多样性研究  

Genetic Diversity of Inbred Lines for Temperate of North America Hybrids in Maize

作　　者：王安贵 陈泽辉 李娟 祝云芳 郭向阳 赵丽[1,3] 胡兴[1,3] 

机构地区：[1]贵州省旱粮研究所,贵阳550006 [2]贵州省现代中药材研究所,贵阳550006 [3]贵州大学农学院,贵阳550025

出　　处：《玉米科学》2014年第1期7-14,共8页Journal of Maize Sciences

基　　金：贵州省科技农业攻关"美国先锋种质在南方山区玉米育种中的改良创新及利用潜势研究"[黔科合NY字(2012)3030号];贵州省农业科学院基金项目"美国先锋玉米杂交种的选系研究"[黔农科合(基金)2010024]

摘　　要：利用SSR标记对北美温带玉米杂交种选育的24个自交系和我国生产应用的10类骨干代表系进行遗传多样性评价。结果表明，54对SSR引物在48份玉米自交系间共检测到290个等位基因变异，每对引物检测出3～9个等位基因，平均为5．4个。每个位点的多态性信息量（PIC）变化于0．29～0．86之间，平均为0．70。标记索引系数（MI）变化范围0．86～7．45，平均为3．86。供试北美温带选系和其他类群的玉米种质均具有较丰富的遗传多样性。3个杂交种选系的遗传相似系数中3号选系最小（0．776），说明3号较其余两个杂交种选系的遗传差异大。根据SSR标记聚类分析、主坐标分析和遗传进化分析结果表明，北美温带选系和其他种质的48份玉米自交系划分为6大类群，分类结果与系谱来源基本一致。A total of 290 alleles were scored between 48 maize inbred lines, averaging 5.4 and ranging from 3 to 9 alleles per locus. PIC values ranged from 0.29 to 0.86 with an average of 0.70. Alleles index averaged 3.86, with a range of 0.86 to 7.45. The amplitude of variation of three genetic diversity index values were larger and higher than the reported in previous similar studies. This results indicated that the genetic diversities of the inbred lines for temperate of North America hybrids and others germplasms were abundant. The similarity coefficient of three hybrids of genetic 3 inbred lines minimum （0.776）, description of 3 as compared with the other two kinds of hybrid genetic difference of large. According to SSR molecular marker clustering analysis, principal coordinate analysis and genetic evolution analysis results showed that the vanguard line and other collections of 48 maize inbred lines were divided into six groups of classification results agreed with the available pedigree data.

关 键 词：玉米 北美温带自交系 杂交种 遗传多样性 

分 类 号：S513.035.1[农业科学—作物学]