外周血sCD14和sVCAM-1在脓毒症新生儿心肌损害时的变化及意义  

Changes and Significences of the peripheral blood sCD14 and sVCAM-1 in the Myocardial injury of Neonatal sepsis

作　　者：徐灵敏[1,2] 孙琪青[3] 邵斌[4] 贾莉婷[5] 张展[5] 

机构地区：[1]复旦大学附属中山医院青浦分院 [2]复旦大学附属儿科医院综合儿科,上海201000 [3]河南省郑州市儿童医院心内科,河南郑州450000 [4]郑州大学第三附属医院药剂科,河南郑州450052 [5]郑州大学第三附属医院检验科,河南郑州450052

出　　处：《中国临床医生杂志》2014年第1期37-39,共3页Chinese Journal For Clinicians

基　　金：河南省医学卫生重点科技资助项目(200802007)

摘　　要：目的研究脓毒症新生儿心肌损害（MI）时外周血sCD14和sVCAM-1的变化及意义。方法选取脓毒症新生儿50例，其中MI20例为脓毒症MI组，其余30例为脓毒症组；同期正常新生儿20例为对照组。所有研究对象均留取1～1．5ml抗凝全血分离血浆，采用酶联免疫双抗体夹心（ABC-ELISA）法检测血浆sCD14和sVCAM-1的浓度。结果sCD14和sVCAM-1的表达水平在新生儿脓毒症MI组均最高，与脓毒症组和对照组的比较，差异有显著性，两者在新生儿脓毒症组的表达水平也显著高于对照组（F=43．538，P〈0．05：F=170．237，P〈0．05）。结论CD14和VCAM-1参与了新生儿脓毒症MI的炎症损害过程，动态观察，有助于早期发现和预测脓毒症新生儿MI的发生。Objective To investigate Changes and Significences of the peripheral blood sCD14 and sVCAM-1 in the Myocardial injury of Neonatal sepsis. Method sCD14 and sVCAM-1 levels were measured by using double-antibody sandwich enzyme-linked immunosorbent （ABC-ELISA） method in 20 cases with MI in neonatal sepsis, 30 eases with mild neonatal sepsis and 20 healthy neonates. Result The plasma sCD14 and sVCAM-1 levels in the sepsis-MI group were all the highest among the three groups, statistically different with those in the other two groups, and the plasma levels in the mild neonatal sepsis group were also higher than those in the control group （F = 43. 538, P〈 0. 05; F= 170. 237 ,P〈0.05）. Conclusion CD14 and VCAM-1 may be involved in the pathogenesis of neonatal sepsis and its MI. Dynamic observation of the changes of the peripheral blood sCD14 and sVCAM-1 levels could be used to diagnosis early and evaluate the prognosis of neonatal sepsis and MI.

关 键 词：新生儿 脓毒症 心肌损害 CD14 血管细胞粘附分子-1(VCAM-1) 

分 类 号：R714.3[医药卫生—妇产科学]