实时细胞电子分析技术的应用研究进展  

Research Progress in Application of Real-Time Cell-based Assay(RTCA)

作　　者：严国俊[1] 裴燕芳 朱贞宏 吴洁颖 潘金火[1] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]艾森生物科学公司,圣地亚哥ca92121 [3]艾森生物(杭州)有限公司,杭州高通量新药筛选中心,杭州310030

出　　处：《中国药学杂志》2014年第3期169-173,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81202922);江苏高校优势学科建设工程资助项目(2011ZYX3-004)

摘　　要：目的综述实时细胞电子分析技术的研究进展,为中药研究提供参考。方法根据近年来国内外相关文献,对实时细胞电子分析技术的原理、特点及其应用进行归纳总结。结果与结论实时细胞电子分析技术是一种无标记、无侵害的实时细胞电子分析方法。综述了实时细胞电子分析技术的应用研究进展,为其在药学领域进一步应用发展提供参考,并对其在中药及复方领域的应用前景进行了展望。OBJECTIVE To provide reference for further application of RTCA in the field of TCM （ traditional Chinese medicine） development, and summarize the RTCA technology application progress in recent years. METHODS The RTCA technology princi- ples, characteristics and applications are summarized based on referring to the related literature in recent years. RESULTS AND CONCLUSION RTCA is a kind of unmarked, non-invasive real-time cell electronic analysis method. It is recently used in drug screening, toxicology evaluation, cell growth, division and death mechanism research. The dynamic real-time analysis of cell is more and more concerned. The RTCA technology application prospects in the field of traditional Chinese medicine and compound are demon- strated. It is hoped to provide reference for further application of RTCA in the field of pharmaceutical development.

关 键 词：实时细胞电子分析技术 应用进展 中药 药学 

分 类 号：R917[医药卫生—药物分析学]