红景天苷对人肝癌HepG2细胞增殖及凋亡的影响  

Effects of Salidroside on Cell Proliferation and Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells

作　　者：庞晓斌[1] 谢欣梅[1] 赵清辉[1] 

机构地区：[1]河南大学药物研究所,河南开封475004

出　　处：《中国药学杂志》2014年第3期195-198,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81273652);河南省教育厅自然科学研究项目(2009B350001)

摘　　要：目的观察红景天苷(salidroside)对人肝癌HepG2细胞增殖和凋亡的影响,探讨其抗肿瘤的可能机制。方法以体外培养的人肝癌HepG2细胞作为研究对象,倒置显微镜观察红景天苷对肝癌细胞形态的影响;采用MTS法检测红景天苷对细胞生长的抑制作用;采用流式细胞仪检测细胞凋亡率;Western blot法检测Bcl-2、Bax、caspase-3、caspase-8的表达。结果红景天苷能显著抑制肝癌细胞HepG2增殖并促进其凋亡,下调细胞Bcl-2表达,上调Bax、caspase-3及caspase-8的蛋白表达量。结论红景天苷在体外对人肝癌HepG2细胞具有显著的抑制增殖和促进凋亡作用,其机制可能与其调节凋亡基因Bcl-2、Bax、caspase-3与caspase-8的蛋白表达有关。OBJECTIVE To investigate the salidroside on proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and its possible mechanisms of anti-tumor. METHODS Cultured HepG2 cells in vitro were used as the research object and their cell morphology were observed by inverted microscope. The inhibitory rate of cell proliferation was measured by MTS assay. The cell apoptosis was determined by flow cytometry.The protein expressions of Bcl-2, Bax, caspase-3 and caspase-8 were detected by Western blot analysis.RESULTS Salidroside could inhibit the proliferation of HepG2 cells and promote its apoptosis. The Bax, caspase-3 and caspase-8 protein expression were increased while Bcl-2 protein expression was decreased in HepG2 cells in a concentration-dependent manner. CONCLUSION Salidroside can inhibit the proliferation and induce the apoptosis of HepG2 cells, and the mechanism may be associated with regulating protein expression of Bcl-2, Bax, caspase-3 and caspase-8.

关 键 词：红景天苷 肝癌HEPG2细胞 增殖 凋亡 

分 类 号：R965[医药卫生—药理学]