不同产地蜂胶改善糖尿病大鼠氧化应激功效的比较研究  

The comparative study on improvement of oxidative stress in rats with diabetes mellitus by propolis from different origin

作　　者：张楠楠[1,2] 吴健全[2] 高蔚娜[2] 韦京豫[2] 蒲玲玲[2] 焦昌娅 郭长江[2] 常红[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070 [2]军事医学科学院卫生学环境医学研究所,天津300050 [3]汤臣倍健股份有限公司营养与健康研究中心,广东广州510620

出　　处：《中国食品卫生杂志》2014年第1期23-26,共4页Chinese Journal of Food Hygiene

基　　金：汤臣倍健营养科学研究基金(TY0111102)

摘　　要：目的比较不同产地蜂胶对糖尿病大鼠氧化应激的改善效果。方法雄性Wistar大鼠,以腹腔注射链脲佐菌素(STZ)建立糖尿病模型,按照血糖水平,分为模型对照组、国产蜂胶1组、国产蜂胶2组、国产蜂胶3组以及巴西绿蜂胶组,10只/组,同一批次的10只正常雄性Wistar大鼠为正常对照组。蜂胶组每天灌胃相应的蜂胶混悬液,模型对照组和正常对照组每天灌胃相同体积的溶剂,灌胃时间持续30 d。干预结束后,测定空腹血糖,取血液、肝脏、肌肉,测定总抗氧化力、还原型谷胱甘肽(GSH)、丙二醛(MDA)水平,以及谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性。结果 4种蜂胶均能降低糖尿病大鼠的空腹血糖。国产蜂胶2能够较好地提高糖尿病大鼠肌肉的总抗氧化力,国产蜂胶3在提高肝脏的总抗氧化力、GSH含量以及肌肉的GSH含量、GSH-Px活性,降低肌肉MDA含量方面效果较好,巴西绿蜂胶在降低血浆MDA含量,提高血浆GSH含量、肝脏SOD活性方面效果较突出。结论 4种蜂胶均能降低糖尿病大鼠血糖水平;4种蜂胶均能有效地改善糖尿病大鼠的氧化应激,但是由于成分的差异,改善的程度有所不同。Objective To compare the effects of propolis from different origin on the antioxidant function in rats with diabetes mellitus. Methods Diabetes mellitus was induced in male Wistar rats by intraperitoneal injection of STZ dissolved in citrate buffer solution. Fifty induced rats were divided into five groups, 10 rats each, according to fasting blood glucose, i. e. model control group, NO. 1 Chinese propolis, NO. 2 Chinese propolis, NO. 3 Chinese propolis and Brazilian propolis. 10 normal male Wistar rats were used as the normal control group. The corresponding propolis suspension was daily administrated for 4 weeks to propolis groups, and the same volume of solvent was administrated to normal and model control groups. The fasting glucose level was measured for each groups at the end of the intervention. Plasma, liver and muscle antioxidant capacity, contents of reduced glutathione, malonaldehyde, and antioxidant enzyme activities, including glutathione peroxidase and superoxide dismutase were measured. Results All the propolis could decrease the serum glucose level of the STZ-induced diabetes mellitus rats and improve the antioxidant function to certain degrees. NO. 2 Chinese propolis could improve the muscle＇s antioxidant capacity of diabetes rats, better than the others. NO. 3 Chinese propolis have a better effect on the DM rats liver＇s antioxidant capacity and contents of glutathione, and could improve the muscle＇s contents of glutathione and glutathione peroxidase＇s activity. The Brazilian propolis can better decrease the contents of serum malonaldehyde, increase the contents of serum GSH, and improve the DM rats liver＇s SOD activity. Conclusion All the four propolis could decrease the serum glucose level and increase the antioxidant capacity at different level by different ways.

关 键 词：糖尿病 大鼠 氧化应激 蜂胶 

分 类 号：R587.1[医药卫生—内分泌]