基于钴铝水滑石/铁氰化钴的无酶葡萄糖传感器的研究  

The study of non-enzymatic glucose sensor based on cobalt-aluminium layered double hydroxide/cobalt hexacyanoferrate

作　　者：杨绍明[1] 孙清[1] 李红[1] 李瑞琴[1] 尚培玲 

机构地区：[1]华东交通大学基础学院化学化工系,南昌330013

出　　处：《分析试验室》2014年第2期148-152,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21065004);江西省教育厅科技项目(GJJ12304)资助

摘　　要：采用电沉积的方法将铁氰化钴（CoHCF）和钴铝水滑石（CoAl-LDH）共同固载于玻碳电极（GCE）表面构筑CoAl—LDI-I／CoHCF纳米材料修饰电极，并利用CoAl．LDH和CoHCF两种物质在碱性环境中对葡萄糖的协同电催化氧化作用，将其用于葡萄糖的分析。采用扫描电镜对电化学沉积的CoHCF和CoAl．LDH的表面形貌进行了表征，利用X射线能量色散谱（EDS）对电沉积的CoAl—LDH／CoHCF复合材料进行了分析。采用循环伏安法（CV）对修饰电极催化氧化葡萄糖的行为进行了研究。利用电流-时间法对CoAl—LDH／GCE，CoHCF／GCE，CoAl-LDH／CoHCF／GCE和裸GCE等4种电极对葡萄糖的催化效果进行了对照。对底液NaOH的浓度和扫速对电极的影响进行了探讨。该传感器对葡萄糖在5．0×10^-7～6．46×10^-4mol／L范围内呈良好的线性响应，相关系数为0．9996，灵敏度为0．13A·L·mol^-1，检出限（S／N=3）为2．1×10^-7mol／L。Through the electrodeposition method, cobalt hexacyanoferrate （CoHCF） and cobalt-aluminium layered double hydroxide （CoAl-LDH） were immobilized on the surface of a glassy carbon electrode for the preparation of CoAI-LDH/CoHCF nanomaterial modified electrode. By the use of the synergistic electrocatalytic effect to glucose, the electrode was exploited to detect glucose. SEM was used to characterize the morphology of CoAl-LDH and CoHCF prepared by eleetrodeposition. X-ray energy dispersive spectroscopy was used to study CoAl-LDH/CoHCF composite. The catalytic oxidation of glucose at the modified electrode was studied by cyclic voltammetry. The catalytic effect of the CoA1-LDH/GCE, CoHCF/GCE, CoA1-LDH/CoHCF/GCE and the bare GCE towards glucose was compared by chronoamperometry （l-t）. The effects of the NaOH concentration and scan rate on the electrode were investigated. The modified electrode shows a good linear range of 5.0 ×10^-7 - 6.46 ×10^-4 mol/L for glucose （ R = 0. 9996 ）, with a detection limit of 2. 1 ×10^-7 mol/L. The biosensor is characterized by high sensitivity, good selectivity and stability.

关 键 词：铁氰化钴 钴铝水滑石 电沉积 葡萄糖 传感器 

分 类 号：O657.1[理学—分析化学]