基于液相色谱联合离子阱-飞行时间质谱技术的海洛因滥用成瘾大鼠血清代谢组学研究  

Metabonomics study of serum in heroin abused rats by liquid chromatography coupled with ion trap-time of flight mass spectrometry

作　　者：吴明健[1] 王玫[2] 杨瑞琴[1] 张大明[2] 

机构地区：[1]中国人民公安大学,北京100038 [2]北京市公安局法医检验鉴定中心,北京100085

出　　处：《分析试验室》2014年第2期183-186,共4页Chinese Journal of Analysis Laboratory

基　　金：公安部重点研究计划项目(2009ZDYJBJSJ001)资助

摘　　要：建立海洛因成瘾动物模型,研究海洛因成瘾大鼠血清代谢物变化,寻找其潜在的标志物。实验动物设成瘾组和空白对照组,运用液相色谱联合离子阱-飞行时间质谱(LC/MS-IT-TOF)分析方法研究海洛因成瘾对大鼠血清代谢变化的影响。分别采用主成分分析(PCA)、偏最小二乘法-判别分析(PLS-DA)分析成瘾组和正常对照组之间的代谢差异。结果表明成瘾组和对照组大鼠在血清代谢水平上具有明显差异,经统计学分析初步筛选出26个潜在的生物标记物的质荷比。海洛因成瘾代谢组学研究具有一定的可行性,代谢组学在判定海洛因成瘾与否方面具有一定的潜在价值。To establish animal model of heroin abused and to study the metabolic changes of serum in heroin abused rats. The animals were randomly divided into the control and model groups. Serum of the control and model groups was analyzed by using LC/MS-IT-TOF. The data were analyzed with principal component analysis （PCA） and partial least squares discriminant analysis （PLS-DA）. The result showed that the distinct differences existed between these two groups on the serum metabolic level, and 26 potential biomarkers＇ s rn/z were found in serum using multivariate statistical analysis . The metabonomics research of heroin abused was feasible which demonstrated the potential value of metabonomics in heroin abused.

关 键 词：海洛因 代谢组学 液相色谱联合离子阱-飞行时间质谱 

分 类 号：O657.63[理学—分析化学]