虎潜丸对去卵巢大鼠骨密度和TGF-β2表达的影响  

Effects of Huqian pills on bone mineral density and TGF-β2 expression in ovariectomized rats

作　　者：张紫铭 肖四旺[2] 吴官保[3] 陈孟交[2] 朱建华[1] 谢义松[2] 

机构地区：[1]湖南中医药大学 [2]湖南中医药大学第二附属医院,湖南长沙410005 [3]湖南省中医药研究院附属医院,湖南长沙410006

出　　处：《湖南中医杂志》2014年第1期112-114,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省科技厅科研基金项目(编号:S2011F1023)

摘　　要：目的:观察虎潜丸对去卵巢大鼠骨密度和转化生长因子-β2(TGF-β2)的影响,探讨"肾主骨"理论与去卵巢大鼠模型骨密度和TGF-β2的相关性。方法:将40只SD大鼠按随机原则分为虎潜丸组、雌激素组、模型组、假手术组,每组10只。对除假手术组外的其余3组大鼠行卵巢摘除手术,术后7d各组予相应药物灌胃治疗。干预12周后,观察比较各组大鼠离体第5腰椎骨骨密度和大鼠腰椎松质骨、肾组织中TGF-β2的表达强度。结果:虎潜丸组和雌激素组大鼠骨密度、腰椎骨及肾组织中TGF-β2表达水平均高于模型组,差异有统计学意义(P<0.01,P<0.05)。结论:虎潜丸能提高去卵巢大鼠模型腰椎骨骨密度,并上调腰椎松质骨、肾组织中TGF-β2的表达。Objective:To observe the effects of Huqian pills on the bone mineral density(BMD) and transforming growth factor β2(TGF-β2) expression in ovariectomized rats and to investigate the association of theory of ' kidney governing bones' with the BMD and TGF-β2 in the ovariectomized rat model.Methods:Forty SD rats were randomly divided into Huqian pill group(n = 10),estrogen group(n = 10),model group(n = 10),and sham operation group(n = 10).All rats except those in the sham operation group were ovariectomized,and all groups received respective drugs by gavage at 7 d after operation.After 12 weeks of intervention,these groups were compared in terms of the BMD of the fifth lumbar vertebra in vitro and the expression of TGF-β2 in the cancellous bone of lumbar vertebrae and renal tissue.Results:The Huqian pill group and estrogen group had significantly higher BMD of the fifth lumbar vertebra in vitro and expression of TGF-β2 in the cancellous bone of lumbar vertebrae and renal tissue than the model group(P < 0.01 or P < 0.05).Conclusion:Huqian pills can increase the BMD of lumbar vertebra and up-regulate the expression of TGF-β2 in the cancellous bone of lumbar vertebrae and renal tissue.

关 键 词：骨质疏松症 虎潜丸 骨密度 TGF-Β2 实验研究 

分 类 号：R285.5[医药卫生—中药学]