淫羊藿素抑制人脐静脉内皮细胞血管生成及其机制  

The Suppressive Effect of Icaritin on Angiogenesis of Endothelial Cells in Human Umbilical Vein and Its Associated Mechanisms

作　　者：吕祥[1,2] 冯彦军[3] 夏英[2] 刘静[1] 侯风刚[1] 李雁[1] 任建琳[1] 陈伟杰[4] 

机构地区：[1]上海市中医医院肿瘤科,上海200071 [2]上海市中医医院药物临床试验机构办公室,上海200071 [3]苏州市第五人民医院肿瘤科,苏州215007 [4]上海市中医医院泌尿外科,上海200071

出　　处：《世界中医药》2014年第1期71-74,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(编号:81173221);国家中医药重点专科建设项目(编号:ZJ0901ZL020);上海市中医肿瘤特色专科建设(编号:2008YSZK008);上海市卫生局科研项目(编号:20124Y018);上海市教委创新项目(编号:12YZ057);上海中医药大学校级项目(编号:2009050)

摘　　要：目的:探讨淫羊藿素对人脐静脉内皮细胞(HUVEC)血管生成的抑制作用及其机制。方法:体外培养人脐静脉内皮细胞,分别观察淫羊藿素对其增殖、迁移及其小管形成的影响。采用酶联免疫吸附法检测血管内皮细胞生长因子(VEGF)和色素上皮衍生因子(PEDF)的含量。结果:经淫羊藿素作用48 h后,未见对内皮细胞增殖有影响。淫羊藿素高浓度组(ICT1 10-6mol/L)、中浓度组(ICT2 10-7mol/L)、低浓度组(ICT3 10-8mol/L)和沙利度胺(TLD)组HUVEC细胞迁移数目(4.67±1.26)、(10.48±3.15)、(21.06±6.83)和(19.15±6.03)个,明显低于空白对照(Control)组(41.38±7.78)个(P<0.01)。ICT1、ICT2、ICT3及TLD组小管形成的面积分别为(5 867.45±925.36)、(1 627.33±288.56)、(735.73±325.65)和(2 933.24±741.43)μm2/视野,均低于Control组(7 883.69±1 034.85)μm2/视野(P<0.01)。经淫羊藿素处理后,HUVEC细胞分泌VEGF功能明显下降,而分泌PEDF功能明显升高。结论:体外实验结果表明淫羊藿素具有抑制HUVEC细胞血管生成的作用,这种效应与血管内皮细胞VEGF的活性降低及其合成受到抑制,同时对PEDF活性升高及其合成受到促进作用有关。Objective ：To investigate the suppressive effect of Icaritin on angiogenesis of human umbilical vein endothelial ceils （ HU- VEC） and its associated mechanisms. Methods： HUVEC cells were cultured in vitro, and we observed the effects of Icaritin on its prolif- eration, migration and tube formation. The content of vascular endothelial growth factor （VEGF） and pigment epithelium-derived factor （PEDF） was detected by ELISA. Results： Icaritin had no significant effect on the proliferation of HUVEC cells after 48h treatment. The number of migrated ceils in Icaritin high concentration group （ICT1 10-6 mol/1）, middle concentration group （ICT2 10 -7 mol/1）, low concentration group （ ICT3 10 -8 mol/1） and Thalidomide （TLD） group were 4.67 ± 1.26, 10.48 ± 3.15, 21.06 ± 6.83, 19.15 ± 6.03 respectively, and all of which were lower compared with the control group （41.38 ±7.78） （P 〈0. 01 ）. The area of tube formation in ICT1 ,ICT2 ,ICT3 and TLD were separately 5 867.45 ± 925.36, 1 627.33 ± 288.56,735.73 ± 325.65,2 933.24 ± 741.43 μm2 per vi- sion, all of which were lower than the control group （7 883.69 ± 1 034.85 μm2 per vision） （P 〈0.01 ）. After Icaritin treatment, HU- VEC cells had a significant lower ability to secret VEGF, but asfor PEDF, it was on the opposite situation. Conclusion：Icaritin can in- hibit the angiogenesis of HUVEC cells in vitro. Such effect may be related to the decreased activity and synthesis of VEGF in vascular en- dothelial ceils, and also associated with the increased activity and synthesis of PEDF.

关 键 词：淫羊藿素 人脐静脉内皮细胞 血管新生 血管生成抑制剂 

分 类 号：R285.5[医药卫生—中药学]