不同培养液对小鼠受精卵体外发育的影响  

Effects of culture media on mouse oosperm growth in vitro

作　　者：王辉田[1] 罗光彬[1] 陈思[1] 孙超[1] 

机构地区：[1]沈阳农业大学大学畜牧兽医学院,辽宁沈阳110866

出　　处：《畜牧与兽医》2014年第1期32-36,共5页Animal Husbandry & Veterinary Medicine

摘　　要：小鼠卵母细胞体外受精后通过比较4种培养液(M16、mCZB、HTF、mCZB*)对小鼠受精后胚胎发育的影响,以期建立最优的体外受精培养液系统。结果表明:受精卵在mCZB*、M16、mCZB、HTF培养液中2-4细胞发育率分别为60.26%、50.00%、48.72%和52.78%。mCZB*比其他3种培养液效果显著(P<0.05),其他3个培养液之间差异不显著(P>0.05)。在mCZB*、M16、mCZB、HTF培养液中囊胚发育率分别为44.37%、27.03%、26.28%和22.22%,各培养液处理组之间差异显著(P<0.05)。本试验提示mCZB*为小鼠体外受精胚胎发育的最佳培养液。The present study were designed to compare the effect of four culture media（ M16,mCZB,HTF and mCZB＊） on the develop- ment competence of mouse oosperm in vitro,for the establishment of efficient culture system in vitro fertilization. The results showed that em- bryonic development of 2- 4 cell rates were 60. 26%,50. 00%,48. 72% and 52. 78%,respectively,when the mouse oosperm was cultured in the culture fluids of M16,mCZB,HTF and mCZB＊. The embryonic development of 2- 4 cell stage in the mCZB＊group was significantly different from other three groups（ P 0. 05）,but,there were no significantly differences among each other M16,mCZB and HTF treated groups（ P 0. 05）. The embryonic development of blastocyst rates were 44. 37%,27. 03%,26. 28% and 22. 22%,respectively,and showed significantly differences between each other（ P 0. 05）. Our results suggested that mCZB＊was the best culture media for the deve- lopment of mouse embryos in vitro fertilization.

关 键 词：小鼠 培养液 受精卵 体外发育 

分 类 号：S814.8[农业科学—畜牧学]