邻苯二甲酸二乙基己酯长期暴露对鲤鱼粘液细胞的影响  

Effect of Long-term DEHP Exposure on Mucous Cells of Cyprinus carpio Linnaeus

作　　者：张贵生[1] 

机构地区：[1]菏泽学院生命科学系,山东菏泽274015

出　　处：《四川动物》2014年第1期127-131,共5页Sichuan Journal of Zoology

基　　金：山东省自然科学基金项目(ZR2010CM046);山东省十二五动物生理生化与应用重点实验室资助项目

摘　　要：利用阿利新蓝和过碘酸雪夫氏(AB-PAS)染色方法,研究了邻苯二甲酸二乙基己酯(DEHP)对鲤鱼鳃及消化道粘液细胞密度的影响,实验分为空白对照组、吐温80组及5 mg/L、20 mg/L、80 mg/L、160 mg/L DEHP组,染毒20 d。结果表明,吐温80组与空白对照组相比差异不明显。除5 mg/L组口腔上皮处粘液细胞密度显著低于吐温80组外,在实验剂量范围内,鳃丝、口腔上皮、前肠、后肠粘液细胞密度均极显著低于吐温80组。中肠处粘液细胞密度5 mg/L、20 mg/L组与吐温80组相比差异不显著,80 mg/L组显著低于吐温80组,160 mg/L组极显著低于吐温80组。鳃丝、口腔上皮、中肠处粘液细胞密度均随DEHP浓度的增大而减小,而前肠和后肠在80 mg/L组粘液细胞密度最小,降幅最大。实验结果还表明:在实验剂量范围内,与吐温80组相比,DEHP能降低鳃丝、口腔上皮处Ⅱ、Ⅳ型,前肠Ⅰ、Ⅱ、Ⅳ型,中肠Ⅰ、Ⅲ、Ⅳ型,后肠Ⅱ、Ⅲ型粘液细胞密度。DEHP能提高鳃丝Ⅰ、Ⅲ型,口腔上皮和前肠Ⅲ型,中肠Ⅱ型和后肠Ⅰ型粘液细胞密度。DEHP能减小鳃丝及消化道粘液细胞的密度,对各类型粘液细胞密度的影响因部位不同、DEHP浓度不同而异。The mucous cell types of digestive tract and gill of Cyprinus carpio Linnaeus were classed by alcian blue and pe- riodic acid schiff staining （AB-PAS）, and the effect of di-（2-ethylhexyl） phthalate （DEHP） on mucous cell density of these tissues were studied. Six groups including blank control, tween-80 control, and 5 mg/L, 20 mg/L, 80 mg/L, 160 mg/L DEHP groups treaded for 20 days were used. The result showed that there was no significant difference between the tween-80 control and the blank control. In the range of tested experimental doses, the mucous cell densities of gill filament, oral epithelium, foregut and hindgut were decreased significantly compared with the tween-80 control group （P 〈 0.05 ）. The mucous cell densities of gill filament, oral epithelium and middle intestines were decreased with the increasing of the concentration of DEHP, and the lowest densities were observed in foregut and hindgut of 80 mg/L DEHP treated group. It also showed that after immersing stimulated with DEHP, the densities of type 11 and IV mucous cell in oral epithelium, gill filament, type Ⅰ, Ⅱ , Ⅳ in former intestines, type Ⅰ, Ⅲ, Ⅳ in middle intestines and type Ⅱ, Ⅲin retrial intestines were all decreased. However, the densities of type Ⅰ , Ⅲ mucous cell in gill filament, type Ⅲ in oral epithelium and for- mer intestines, type Ⅱ in middle intestines and type Ⅰ in retrial intestines were increased. In conclusion, DEHP can re- duce the density of mucous cells in digestive tract and gill filament. The effect of DEHP on the density of mucous ceils is correlated with the part of digestive tract and concentration of DEHP.

关 键 词：邻苯二甲酸二乙基己酯 鲤鱼 粘液细胞 

分 类 号：S943[农业科学—水产养殖]