羟基磷灰石/聚醚酯聚氨酯支架的体外降解行为和细胞相容性研究  

Degradation behavior and cell biocompatibility in vitro of hydroxyapatite/poly(ether-ester urethane)scaffold

作　　者：刘浩怀[1] 张建华[1] 许庆陵[1] 刘鹏[1] 何芝洲[1] 

机构地区：[1]广州大学化学化工学院分析测试中心,广州510006

出　　处：《化工新型材料》2014年第1期86-89,共4页New Chemical Materials

基　　金：国家自然科学基金项目(51203028;21106023);广东省自然科学基金资助项目(S2011040004038);广东高校优秀青年创新人才培养计划项目资助(LYM11097);广州市科技计划项目资助(12C32011650)

摘　　要：采用XRD、DSC、体外降解实验和细胞相容性实验等方法对羟基磷灰石/聚醚酯聚氨酯(HA/PU)多孔支架的结构和性能进行了研究。结果表明,HA粒子添加到聚醚酯聚氨酯基体中,在一定程度上降低了聚氨酯软段的结晶,提高了聚氨酯基体的力学性能。体外降解实验表明HA/PU复合支架的降解不会引起浸泡液pH值较大的波动,且降解初期的力学性能衰减缓慢。MG63细胞与HA/PU复合支架共培养的实验表明,细胞生长良好,牢固地黏附在支架表面,并在支架表面充分伸展,复合支架具有良好的细胞相容性。这些结果表明HA/PU支架有望用于骨组织工程修复。The properties of hydroxyapatite/poly（ether-ester urethane） （HA/PU） scaffold were characterized by XRD, DSC, degradation in vitro and biocompatihility tests. The results indicated that the incorporation of HA particles in poly（ether-ester urethane） can hinder the crystallization process of soft segment, and improved the mechanical properties of PU matrix： The degradation in vitro experiments showed that the degradation of HA/PU scaffold can not cause large fluctuations in pH value of the soaking solution, and the mechanical properties decaied slower in the initial degradation. The biocompatibility in vitro tests showed that the cells were adhered well to the surface of the scaffolds, and exhibited normal, well-developed filopodia and lamelliopodia morphology. These results indicated that the porous HA/PU scaffold may be a prospective scaffold material for future applications.

关 键 词：聚醚酯聚氨酯 支架 降解 细胞相容性 

分 类 号：TB383.4[一般工业技术—材料科学与工程]