一种免疫抑制药物体外筛选模型的建立及评价  

Establishment and evaluation of a screening model for immunosuppresives in vitro

作　　者：李覃[1] 曹波[2] 高颖[2] 王伟[3] 陈虹[2] 

机构地区：[1]武警后勤学院临床医学系病原生物与免疫学教研室,天津300309 [2]武警后勤学院救援医学系生药与药剂学教研室,天津300309 [3]武警后勤学院附属医院,天津300162

出　　处：《武警后勤学院学报（医学版）》2013年第12期1056-1059,共4页Journal of Logistics University of PAP（Medical Sciences）

基　　金：国家自然科学基金(81202843);中国博士后科学基金特别资助(201104796);天津市应用基础与前沿技术研究计划(11JCYBJC14600)

摘　　要：【目的】建立一种可靠的体外筛选模型评价药物的免疫抑制活性。【方法】在以二硝基氟苯(2,4-dinitrofluorobenzene,DNFB)诱导小鼠耳廓接触性超敏反应(contact hypersensitivity,CHS)模型的基础上,体外培养模型小鼠脾脏T细胞,首先分析细胞增殖活性,进而采用RT-PCR分析核因子IFN-γ、IL-4、T-bet、GATA-3的mRNA表达,最后通过三种临床上常用的免疫抑制药物进行验证。【结果】模型小鼠T淋巴细胞在体外能维持一定时程的高反应性,IFN-γ/IL-4与T-bet/GATA-3比值均明显增加,T细胞向Th1方向分化偏移,同时,环孢素、地塞米松、霉酚酸酯均能显著抑制T细胞的体外增殖。【结论】本研究初步建立了一种简单的可用于筛选免疫制剂的体外细胞模型。[ Objective] To make a simple and reliable screening model in vitro to investigate the effect of immunosuppressive agents. [ Methods ] DNFB induced CHS mouse model was used. T lymphocytes of the CHS mouse were prepared. Then, proliferation analyse and semi-quantitative RT-PCR were used to explore the characteristics of T lymphocytes from CHS mice. [Results] The T lymphocytes of CHS mice could maintain the higher reactivity in vitro for a period of time, accompanied with the increase of IFN-γ/IL- 4 and T-bet/GATA-3. Furthermore, three kinds of immunosuppressive drugs were proved to inhibit the proliferation of T lymphocytes from CHS mice in vitro. [Conclusion] It＇s suggested that an easy and feasible screening model for immnnosuppressive agents in vitro might he established preliminarily.

关 键 词：免疫抑制剂 细胞培养 体外筛选 T淋巴细胞 模型 

分 类 号：R967[医药卫生—药理学]