实时荧光定量PCR检测血液标本中光滑念珠菌方法的建立  

Real-time quantitative PCR for detection of blood Candida glabrata

作　　者：孔丹莉[1] 周浩[2] 王茜[2] 艾云灿[3] 何玉清[1] 刘伟伟[1] 杨杰[1] 丁元林[1] 

机构地区：[1]广东医学院公共卫生学院流行病与卫生统计教研室,广东东莞523808 [2]南方医科大学附属南方医院,广东广州510515 [3]中山大学生命科学学院,广东广州510275

出　　处：《广东医学院学报》2013年第5期493-495,共3页Journal of Guangdong Medical College

基　　金：广东省自然科学基金面上项目(No.S2012010008271);东莞市科技计划高等院校项目(No.2012108102058);东莞市医疗卫生重点项目(No.2012105102010);广东医学院2011年博士启动基金(No.XB1212)

摘　　要：目的探索快速可靠检测血液标本中光滑念珠菌的新方法。方法采用真菌26S rDNA D1/D2区序列作为引物,单独和同时扩增光滑念珠菌及白色念珠菌与光滑念珠菌混合物,对血液标本进行荧光定量检测。结果本方法不仅能够稳定的扩增出光滑念珠菌26S rDNA序列,并且能准确区分出混合物中的白色念珠菌与光滑念珠菌。结论应用26S rDNAD1/D2区序列的荧光定量PCR是快速、敏感和特异的检测光滑念珠菌方法。Objective To investigate a rapid and reliable method for detection of blood Candida glabrata. Methods Real- time quantitative PCR was applied to detect C. glabrata and mixture of C. glabrata and C. albicans from blood using fungal primers D 1/D2 region of 26S rDNA. Results D 1/D2 region of 26S rDNA was stably amplified in C. glabrata, and the products of C. glabrata and C. albicans were dist/nguishable. Conclusion Real-time quantitative PCR using D 1/D2 region of 26S rDNA is a rapid, sensitive and specific method for detection of C. glabrata.

关 键 词：光滑念珠菌 白色念珠菌 荧光定量PCR 

分 类 号：R379.4[医药卫生—病原生物学]