重组人白介素-10对兔皮肤移植排斥反应及外周血T细胞亚群的影响  

Effect of recombinant human IL-10 on skin grafting rejection and peripheral blood T lymphocyte subsets in rabbits

作　　者：温琼娜[1] 夏嫱[1] 张磊[1] 刘凯山[1] 孙万邦[1] 

机构地区：[1]遵义医学院珠海校区/贵州省免疫学研究生教育创新基地,广东珠海519041

出　　处：《广东医学院学报》2013年第5期504-506,524,共4页Journal of Guangdong Medical College

基　　金：贵州省教育厅自然科学重点培育项目(No.黔2008032)

摘　　要：目的观察重组人白介素-10(rhIL-10)对白兔皮肤移植排斥反应及外周血T细胞亚群的影响。方法 36只白-1-1兔随机等分为供体组和受体组,再将18只受体白兔随机分为rhIL-10组(5、10 g·kg·d)、环孢素(CsA)阳性对照组(5、-1-1-1-1-1-110 mg·kg·d)、rhIL-10+CsA联合组(5 g·kg·d hIL-10+5 mg·kg·d CsA)、生理盐水阴性对照组(1 mL/d)。在移植++前1 d至术后8 d连续肌注药物,观察各组皮片移植排斥反应情况及外周血CD4和CD8细胞数、CD4/CD8细胞比值。结果rhIL-10组排斥反应发生时间比阴性对照组延长(P<0.05),而rhIL-10+CsA组排斥反应发生时间比低剂量rhIL-10及+CsA组延长(P<0.05)。在发生急性排斥反应时,CD4细胞数及CD4/CD8细胞比值升高,在皮片坏死时达到峰值。结论 rhIL-10可抑制白兔皮肤移植排斥反应,CD4/CD8细胞比值有助于监测排斥反应发生。Objective To investigate the effect of recombinant human IL-10 （rhIL-10） on skin grafting rejection and peripheral blood T lymphocyte subsets in rabbits. Methods Thirty-six white rabbits were equally divided into donor and recipient groups, and the recipient group was further allocated to rhIL-10 group （5 μg .kg-1.d-1 and 10 μg . kg-1. d-1）, cyclosporin （CsA） group （5 mg .kg-1. d-1 and 10 mg .kg-1. d-1）, rhIL-10 （5 μg .kg-1 .d-1） ＋ CsA （5 mg. kg-1. d-1） group, and normal saline group （I re.L/d）. The agents were continuously injected muscularly from 1 d pre-transplant to 8 d post-transplant. Skin graft rejection, and CD4＋ and CD8＋ cells and CD4/CD8 ratio from peripheral blood were determined. Results Onset of skin rejection was later in rhIL-10 group than that in normal saline group （P〈0.05）, and so did in rhIL-10 ＋ CsA group compared with low-dose rhIL-10 and CsA groups （P〈0.05）. CD4＋ cells and CD4/CD8 ratio increased during skin rejection, and peaked during skin graft necrosis. Conclusion rhIL-10 can inhibit the skin rejection in white rabbits, and CD4/CD8 ratio is useful for monitoring the skin rejection.

关 键 词：白介素-10 兔 皮肤移植 淋巴细胞 

分 类 号：R392.4[医药卫生—免疫学]