福建株白纹伊蚊经口感染登革2型病毒研究  

Study on oral infection of dengue virus type 2 in Aedes albopictus from Fujian

作　　者：肖方震[1] 邓艳琴[1] 谢汉国[1] 何似[1] 

机构地区：[1]福建省人兽共患病研究重点实验室(福建省疾病预防控制中心),福州350001

出　　处：《海峡预防医学杂志》2013年第6期7-9,共3页Strait Journal of Preventive Medicine

基　　金：福建省卫生厅青年科研课题(2011-2-26)

摘　　要：目的探讨福建株白纹伊蚊对登革2型病毒的易感性。方法用C6/36细胞培养登革2型病毒,白纹伊蚊人工经口感染病毒混合液,14d后分别用间接免疫荧光和RT-PCR检测蚊体内的登革2型病毒。结果用间接免疫荧光检测感染登革2型病毒的白纹伊蚊,福建株白纹伊蚊感染率为44.6%(25/56),头部感染率为32.1%(18/56);用RT-PCR方法扩增出511bp片段,感染率为53.9%(14/26)。结论福建株白纹伊蚊对登革2型病毒易感。Objective To study the susceptibility of Ae. albopictus to dengue virus type 2 from Fujian. Methods Dengue virus type 2 （DENV2） were cultured with C6/36 cells, Ae. albopictus were infected with micebloodsugar virus suspension through oral. The antigen virus in body of mosquitoes was detected by indirect immunofluorescence assays （IFA） and RTPCR methods respectively after 14 days postinfection. Results The infection rates of DENV2 in thoraxabdomen and head were 44.6%（25/56） and 32.1%（18/56） respectively detected by using IFA methods. When the fragment of 511bp was produced, the infection rate was 53.9% detected by RTPCR method. Conclusion Ae.albopictus isolates collected are susceptible to DENV2 in Fujian.

关 键 词：白纹伊蚊 登革病毒 福建省 

分 类 号：R373.3[医药卫生—病原生物学] R512.8[医药卫生—基础医学]