根皮苷对日本血吸虫感染小鼠肝纤维化的抑制作用  

INHIBITORY ACTION OF PHLORIDZIN ON HEPATIC FIBROSIS INDUCED BY SCHISTOSOMA JAPONICUM IN MICE

作　　者：杜幼芹[1] 刘英[1] 汪鋆植[2] 冯天艳[2] 邓改改[2] 龚权[3] 

机构地区：[1]三峡大学医学院人体寄生虫学教研室,湖北宜昌443002 [2]天然产物研究与利用湖北省重点实验室,三峡大学,湖北宜昌443002 [3]长江大学医学院,湖北荆州434023

出　　处：《寄生虫与医学昆虫学报》2013年第4期213-217,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：湖北省教育厅产学研合作项目（D20081302）

摘　　要：探讨根皮苷对日本血吸虫感染所致小鼠肝纤维化的保护作用。随机将未感染和感染日本血吸虫尾蚴昆明小鼠分成4组A—D，每组10只：未感染空白对照组（A）、模型组（B）、复方鳖甲软肝片阳性组（C）、根皮苷治疗（40mg/（kg·d））组（D）。感染后第42d，B、C、D组均用吡喹酮驱虫治疗500113tg／（kg·d），2日疗法，之后各组开始灌胃给药持续治疗60d，A和B组生理盐水灌胃对照。实验结束处死小鼠，留取肝脏组织和血清，测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、透明质酸（HA）含量，计算肝体指数，测定肝组织中羟脯氨酸（HYP）含量。苏木素-伊红和Masson胶原纤维染色观察小鼠肝组织变性、虫卵肉芽肿与胶原沉积等病理改变。结果显示，与A组相比，B、C、D组小鼠肝脏有明显虫卵肉芽肿形成、胶原沉积与肝纤维化，伴有不同程度肝细胞炎性损伤坏死。血清中ALT、AST、HA和肝组织中HYP含量均明显升高（P〈0．05或P〈0．01）。与B组相比，根皮苷组ALT、AST、HA和HYP含量和肝体指数均明显降低（P〈0．05或P〈0．01），肝组织坏死与胶原沉积明显减轻，虫卵肉芽肿面积减小。结果表明根皮苷具有抗血吸虫性肝纤维化的作用。To investigate the effects of phloridzin on hepatic fibrosis in mice induced by Schistosoma japonicum, the mice model of hepatic fibrosis which infected by cercariae of S. japonicum wer~ randomly divided into 3 groups： group B as model, group C was positive control by complex liver soften tablet from turtle, group D was treated by 40 mg/ （kg · d）of phloridzin, group A was blank control. Every group had 10 mice. Each grouP Of C, D was orally given praziquantel 1 mL （500 mg/ （kg· d） × 2 d） on 42 d after infection, then began to administration for 60 d. Group A and B were orally given by Sodium Chloride as control. Serum hyaluronic acid （HA）, lanine aminotransferase （ALT） and aspartate aminotransferase （AST）, and hydroxyproline in liver tissues of the mice was detected. Area of egg granuloma and degree of hepatic fibrosis were also observed via HE and Masson staining. Comparing with the blank control group A, the obviously egg granuloma appear, the collagen deposit and fibrosis occur in liver tissues of group B, C, D, the levels of ALT, AST, HA in blood serum and HYP in liver tissues were significantly higher. But the level of ALT, AST, HA and HYP in phloridzin was significantly lower than the model group B, the area of egg granuloma, the collagen deposit and the degree of hepatic fibrosis in group C and D were significantly lower than the model group B. The phloridzin was shown obvious inhibitory action on the hepatic fibrosis induced by S. iaponicum infection.

关 键 词：根皮苷 日本血吸虫 肝纤维化 小鼠 

分 类 号：S816.4[农业科学—饲料科学]