柚皮素对人肺成纤维细胞表达血管内皮生长因子的影响  

The Effect of Naringenin on Vascular Endothelial Growth Factor Released by Human Fetal Lung Fibroblasts

作　　者：徐宪韬 孙丽华[1] 史莹[1] 

机构地区：[1]南京医科大学附属南京医院,江苏南京210006

出　　处：《中国呼吸与危重监护杂志》2014年第1期18-20,共3页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家自然科学青年基金(编号:81100010)

摘　　要：目的探讨柚皮素对人肺成纤维细胞生成血管内皮生长因子(VEGF)的影响,并研究其可能的机制。方法培养人胚肺成纤维细胞,分为对照组、香烟提取物(CSE)组和柚皮素干预组。柚皮素干预组分别与低、中、高剂量的柚皮素共孵育2 h后,与CSE组一起加入CSE,调整终浓度柚皮素5μmol/L、10μmol/L和20μmol/L,CSE终浓度5%。培养24、48及72 h后,ELISA法测定各组VEGF含量,采用Western blot法观察各组Smad3及磷酸化Smad3(p-Smad3)的表达情况。结果在人胚肺成纤维细胞中,柚皮素能抑制CSE诱导的血管内皮生长因子生成。CSE能显著提高p-Smad3水平,而添加了柚皮素后p-Smad3明显减少,并随柚皮素剂量的增加而p-Smad3水平愈低。结论柚皮素能够抑制Smad3的磷酸化,从而抑制CSE诱导的血管内皮生长因子的分泌。Objective To explore the effect of naringenin on the expression of vascular endothelial growth factor （VEGF） released by human fetal lung fibroblasts. Methods Human fetal lung fibroblast cells were divided into a control group, a cigarette smoke extract （CSE） group and a naringenin group. Ceils in the naringenin group were incubated with different doses of naringenin for 2h. Then the naringenin group and the CSE group were incubated with CSE to adjust the final concentration of naringenin （5 μmol/L, 10 μmol/L, and 20 μmoL/L） and of CSE （5%）. The concentration of VEGF was measured in human fetal lung fibroblasts after cultured for 24h, 48h and 72h by enzyme-linked immunoabsorbent assay. Smad3 and p-Smad3 levels were detected by Western blot. Results ELISA results showed that the CSE can significantly increase the VEGF expression, and naringenin can inhibit the increasing of the VEGF expression. Western blot results showed that the CSE can increase the p-Smad3 expression, and the naringenin can inhibit the increasing of the p-Smad3 expression. Conclusion Naringenin can inhibit phosphorylation of Smad3, and decrease the expression of VEGF released by human fetal lung fibroblasts.

关 键 词：柚皮素 香烟提取物 血管内皮生长因子 SMAD3 

分 类 号：R285.5[医药卫生—中药学]