枳抗逆基因PtrZPT2-2的克隆与表达分析  

Cloning and Expression Analysis of PtrZPT2-2 from Trifoliate Orange(Poncirus trifoliata)

作　　者：刘德春[1] 吴启[1] 王玥辰[1] 刘山蓓[1] 刘勇[1] 

机构地区：[1]江西农业大学农学院园艺系,南昌330045

出　　处：《园艺学报》2014年第1期9-16,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31260468);江西省自然科学基金项目(20114BAB214006);江西农业大学科学研究基金项目(CX201101)

摘　　要：通过电子克隆和RT-PCR法从枳[Poncirus trifoliata(L.)Raf.]中克隆到1个C2H2型锌指蛋白家族基因,命名为PtrZPT2-2。该基因编码159个氨基酸残基,预测蛋白分子量为17.28 kD,等电点为9.51。PtrZPT2-2包含两个C2H2型锌指结构域,在其C–末端含有一个转录抑制结构域EAR/DLN-box(DLNL)。PtrZPT2-2与其他植物参与各种非生物胁迫反应的C2H2型锌指蛋白有较高的一致性。系统发育树分析表明PtrZPT2-2与杨树PtaZFP2,矮牵牛ZPT2-12、ZPT2-13,拟南芥ZAT12、ZAT7亲缘关系较近。半定量PCR分析表明,低温、高盐、干旱胁迫和ABA处理下枳叶、茎、根中PtrZPT2-2均被诱导上调。PtrZPT2-2可能在枳适应低温、高盐、干旱等非生物胁迫和ABA响应中起着重要作用。In the present study, a novel C2H2-type zinc finger protein gene, PtrZPT2-2, was cloned from trifoliate orange by in silico and RT-PCR approaches. The PtrZPT2-2 encodes a protein of 159 amino acid residues with a predicted molecular mass of 17.28 kD and an isoelectric point of 9.51. The PtrZPT2-2 protein contains two C2H2-type zinc finger domains, and a putative transcription repression domain （EAR/DLN-box） at the C-terminus. Phylogenetic analysis showed that the PtrZPT2-2 clustered with PtaZFP2, ZPT2-12, ZPT2-13, ZAT12 and ZAT7. PtrZPT2-2 shared high identity with other C2H2-type zinc finger proteins involved in abiotic stress responses. Semi-quantitative PCR analysis showed that expression of PtrZPT2-2 was upregulated by cold, salt, drought and ABA treatments in leaves, stems and roots of trifoliate orange seedlings. Our data suggested that PtrZPT2-2 is a new member of the C2H2-type zinc finger protein genes and may play an important role in responge to cold, salt, drought and ABA stresses in citrus.

关 键 词：装卸工艺 改造 节能 扩容 

分 类 号：S666[农业科学—果树学]