基于EST数据库的葡萄成花途径的预测及分析  

Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database

作　　者：刘丹[1] 慕茜[1] 李晓鹏[1] 刘更森[2] 李玉[1] 张彦苹[1] 房经贵[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]青岛农业大学园艺学院,青岛266109

出　　处：《园艺学报》2014年第1期26-34,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31372027)

摘　　要：以拟南芥成花途径相关基因序列为探针,对葡萄EST数据库进行同源检索筛选,获得相应的同源EST序列,并以葡萄不同发育时期的不同器官cDNA为模板,通过半定量RT-PCR反应研究了葡萄成花途径相关基因的存在与表达情况,对葡萄成花途径的进行了分析与判断。研究发现,葡萄成花途径中的基因在不同器官中的不同时期的时空表达存在强弱的差异。Homologous grape EST sequences were searched against the grape EST database using the corresponding flowering pathway gene sequences from Arabidopsis as the probes. The existence and expression of related genes involved in the flowering pathway in grape were further studied by half-qRT-PCR using cDNA, prepared from different grape organs at various developing stages, as PCR template, bashed on which, the grape flowering pathways were thoroughly forecasted. The study results also indicated that the expression levels of the grape flowering pathway genes showed some spatiotemporal differences.

关 键 词：葡萄 EST 成花途径 基因 

分 类 号：S663.1[农业科学—果树学]