阿氏浮丝藻mcyT基因序列多样性研究  

PHYLOGENETIC STUDY BASED ON MCYT GENE OF PLANKTOTHRIX AGARDHII FROM CHINA

作　　者：潘倩倩[1,2] 朱梦灵[1] 刘洋[1] 林燊[1] 李仁辉[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2014年第1期92-99,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金项目(30970185)资助

摘　　要：为研究我国浮丝藻(Planktothrix Anagnostidis et Komárek)的毒素相关基因,选取分离自我国不同省份水体的13株阿氏浮丝藻,通过PCR检测其微囊藻毒素合成酶基因mcyA、mcyE及mcyT研究其毒素基因特性。PCR结果表明除mcyT之外其他引物检测均无扩增产物,说明这13株浮丝藻不具备产微囊藻毒素的能力。通过克隆测序得到mcyT序列,并进行分子系统分析,构建了关于mcyT序列的Neighbor-Joining系统树,结果表明mcyT序列可以将产毒与不产毒浮丝藻分为两大独立的分支,两个分支之间的最低序列相似度分别为98.5%和99.1%。研究结果可为后续研究我国浮丝藻的微囊藻毒素合成相关基因的多样性以及分子监测提供参考。In this study, thirteen strains ofPlanktothrix agardhiifrom the freshwaters in different provinces were exa-mined to detect the sequences of the synthetase genes of microcystins,includingmcyA,mcyEandmcyT. The PCR re-sults showed that onlymcyT gene was amplified in these strains, indicating that these strains are not microcystin pro-ducers. The phylogenetic analysis based on the mcyTgene demonstrated that thePlanktothrix strains were divided into two clades in the Neighbor-Joining tree, well corresponding to the non-toxic and toxic strains, and the minimum simi-larity of the two clades were 98.5% and 99.1%. The results provided a new insight into the molecular divergence be-tween toxic and non-toxic Planktothrixstrains.

关 键 词：浮丝藻 微囊藻毒素 毒素合成酶基因 mcyT 邻接法 

分 类 号：Q346.5[生物学—遗传学]