白藜芦醇增强人NK细胞杀伤肺癌细胞株A-549的体外研究  

Resveratrol enhances NK cell's cytotoxic activity agianst lung cancer cells A-549 in vitro

作　　者：吕小婷 刘军权 周忠海 王涛[2] 孙蕾清 张颂 童成刚 陈玲 陈复兴 

机构地区：[1]解放军第九七医院中心实验室,徐州221004 [2]南京军区后勤部门诊部,南京210000 [3]解放军第九七医院干部病房,徐州221004

出　　处：《现代免疫学》2014年第1期42-46,共5页Current Immunology

基　　金：南京军区医学科学创新课题(11 MA040)

摘　　要：研究白藜芦醇对人NK细胞体外增殖及杀伤肺癌细胞A-549的影响。用SCGM培养基体外扩增人NK细胞,10 d后用不同浓度的白藜芦醇作用于NK细胞48 h后,MTT法测生长曲线,流式细胞仪检测NK细胞表面穿孔素、颗粒酶B及CD107a的表达;LDH法检测NK细胞对肺癌细胞株A-549的杀伤活性。结果发现,培养10 d后NK细胞纯度达到68%左右,不同浓度白藜芦醇处理NK细胞48 h后,白藜芦醇在浓度0.05-12.5μmol/L可以促进NK细胞的生长,并增加NK细胞表面颗粒酶B、穿孔素及CD107a的表达,对肺癌A-549细胞的杀伤活性在白藜芦醇浓度为0.75μmol/L显著高于对照组。以上实验结果提示,白藜芦醇在一定浓度下能促进NK细胞的生长,且增加对肺癌细胞株A-549的杀伤活性。To investigate the effect of resveratrol on proliferation and cytotoxicity of human NK cells against lung cancer cells A-549 in vitro, NK cells were generated in vitro by stimulating peripheral blood mononuclear cells of healthy donors in SCGM medium for 10 days, then the purity of NK cells was accessed by flow cytometer. After co-cultured with different concentrations of resveratrol for 48 h, growth curve of γδ T cells in each group were determined by MTT assay. Flow cytometry was used to detect expression of perforin, granzyme B, CD107a by NK cells and LDH assay was used to measure eytotoxic activity of NK cells against lung A-549 cells. The purity of NK cells from different individuals cultured for 10 d reached approximately 68%. After treated with various concentrations （0.05-12.5μmol/L） of resveratrol for 48 h, it was found that resveratrol not only promoted NK cells growth but also increased perforin, granzyme B and CD107a expression. Moreover, NK cells treated with 0.75μmol/L of resveratrol showed significantly higher cytotoxicity against A-549 cells than that of the control group. Taken together, resveratrol at proper concentrations could promote proliferation of NK cells and enhance their cytotoxic activity against A-549 cells.

关 键 词：NK细胞 白藜芦醇 杀伤活性 免疫治疗 

分 类 号：R392[医药卫生—免疫学]