软叶针葵花粉疫苗治疗小鼠过敏性鼻炎的效果和机制  

Therapeutic effect and mechanism of Phoenix roebelenii pollen vaccine for treatment of mice with allergic rhinitis

作　　者：蒋聪利 肖小军[2] 马莉[3] 张昕昕[3] 刘志刚[2] 

机构地区：[1]深圳大学生命科学学院 [2]深圳大学医学院过敏反应与免疫学研究所,广东深圳518060 [3]南昌大学基础医学院免疫学教研室,江西南昌330006

出　　处：《细胞与分子免疫学杂志》2014年第2期113-116,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(30070702;811160129);广东省高等学校国际暨港澳台科技合作创新平台项目(2012gjhz0009);深圳市重点实验室组建项目(SW201110010)

摘　　要：目的建立小鼠过敏性鼻炎模型,用重组软叶针葵花粉profilin疫苗免疫预防治疗,观察过敏性鼻炎小鼠的病理变化,探讨该疫苗对过敏性鼻炎治疗效果及可能机制。方法用腹腔注射法建立BALB/c小鼠软叶针葵花粉过敏性鼻炎模型,皮下注射过敏原疫苗进行预防治疗。通过小鼠无创肺功能仪检测小鼠治疗前后气道高反应性、间接ELISA检测血清中特异性抗体(IgE和IgG2a)、细胞因子,透射电镜观察鼻黏膜组织超微结构变化。结果疫苗特异性免疫治疗后,小鼠鼻部症状和气道高反应性减轻,其血清中特异性IgG2a水平升高、IgE水平降低,培养脾细胞上清中INF-γ、IL-10含量增加,IL-4生成减少。结论重组软叶针葵花粉profilin作为疫苗对花粉过敏性鼻炎有一定的疗效,可能与促使Th2向Th1转换,调节辅助性T细胞的平衡有关。Objective To apply Phoenix roebelenfi pollen vaccine to murine models of allergic rhinitis and observe the pathological changes of allergic rhinitis in mice, and to study the efficacy and mechanism of the vaccine for the treatment of allergic rhinitis. Methods BALB/c mice models of allergic rhinitis were established by intrapedtoneal injection, and then treated with immunotherapy of allergen vaccine by subcutaneous injection. The mice were examined for the levels of airway hyperresponsiveness by a noninvasive lung function detector, for the specific antibodies IgE and IgG2a in serum and cytokines by indirect ELISA, and for the pathological changes of ultrastructure of nasal mucosa of the mice by transmission electron microscopy before and after the treatment. Results After the immunotherapy, nasal symptoms and airway hyperresponsiveness of the mice were relieved. The level of specificity antibody IgG2a in serum was elevated, and IgE dropped significantly. In the culture supematant of spleen cells, INF-7. and IL-10 levels increased and the production of IL-4 decreased. Conclusion The recombinant profilin of the Phoenix roebelenii pollen as vaccine has a certain therapeutic effect for the pollen allergic rhinitis, and it works maybe through promoting the transition of Th2 to Th! and regulating the balance of helper T cells.

关 键 词：软叶针葵花粉 过敏性鼻炎 疫苗 超微结构 

分 类 号：R392.11[医药卫生—免疫学] R765.21[医药卫生—基础医学]