肾透明细胞癌组织中树突状细胞与微血管密度之间的关系  

Relationship between dendritic cell and microvessel density in clear cell renal cell carcinoma

作　　者：张亮[1,2] 麦海星[2] 王亚林[2] 赵立[2] 陈立军[2] 张斌[3] 

机构地区：[1]安徽医科大学解放军307医院临床学院 [2]军事医学科学院附属医院泌尿外科 [3]军事医学科学院细胞与基因治疗中心,北京100071

出　　处：《细胞与分子免疫学杂志》2014年第2期191-194,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：吴阶平医学基金(32067521203)

摘　　要：目的探讨树突状细胞(DC)的数量及成熟情况与肾透明细胞癌微血管密度(MVD)的相关性。方法收集解放军307医院2010-07/2013-01期间手术的30例肾透明细胞癌患者术后癌组织及癌旁标本,通过免疫组化检测石蜡切片中DC特异性标志CD11c、DC成熟标志MHC-Ⅱ和MVD标志CD34的表达情况并进行分析。结果 30例肾透明细胞癌患者中,癌组织中CD11c阳性率和MVD分别为(93.08±66.14)‰和26.31±11.05个明显高于癌旁组织(25.91±12.29)‰和12.78±5.49个,癌组织中MHC-Ⅱ阳性率为(9.65±3.61)‰明显低于癌旁组织(17.60±6.26)‰。MVD与MHC-Ⅱ阳性率成负相关关系,而与CD11c则无明显相关性。癌组织中各指标的表达与患者性别、年龄、TNM分期之间未见明显相关性。结论肾透明细胞癌癌组织中的血管密度、DC含量高于癌旁组织,而成熟DC含量低于癌旁组织,肿瘤微血管密度与DC成熟度之间存在负相关关系。Objective To investigate the relationship between the number and maturation of dendritic cell （DC） and microvessel density （MVD） in clear cell renal cell carcinoma （CCRCC）. Methods CCRCC paraffin-embedded tissues and surrounding normal tissues were collected from 30 cases of CCRCC who underwent operations in No. 307 hospital of PLA during July, 2010 to January, 2013. Immunohistochemistry was used to detect the expressions of CDllc, MHC-II, and CD34 to analyze the relationship among them. Results The positive expression rate of CDllc in CCRCC tissues [ （93.08 ＋ 66.14）‰] was higher than that in the surrounding tissues [ （25.91 ± 12.29）‰]. MVD in CCRCC tissues （26.31 ± 11.05） was significantly higher than that in the surrounding tissues （ 12.78 ± 5.49）. The permillage of MHC- II positive DC in CCRCC tissues [ （9.65 ± 3.61）‰] was significantly lower than that in the surrounding tissues [ （17.60 ± 6.26）‰]. There was a negative correlation between the MVD value and the expression of MHC- I1 ; there was no correlation between the MVD and the expression of CDllc. Positive expressions of various indicators in CCRCC tissues were not related to patients＇ age, sex, and TNM classification. Conclusion In CCRCC tissues, there were higher MVD and more DCs than in the surrounding tissues, yet there were less mature DCs. There was a negative correlation between the MVD and the maturation of DC.

关 键 词：肾透明细胞癌 树突状细胞 肿瘤微血管 免疫组化 

分 类 号：R392.33[医药卫生—免疫学] R737.11[医药卫生—基础医学]