慢性乙型肝炎患者中性粒细胞中乙型肝炎病毒的检测和分析  

Detection and analysis of HBV in polymorphonuclear neutrophils of patients with hepatitis B

作　　者：韩忠燕[1] 王健[1] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室

出　　处：《细胞与分子免疫学杂志》2014年第3期299-301,305,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省自然科学基金(1308085MH148;090413138);安徽省教育厅自然基金重点项目(KJ2010A086)

摘　　要：目的探讨乙型肝炎病毒(HBV)能否感染慢性乙肝患者中性粒细胞并在中性粒细胞复制增殖。方法以中性粒细胞分离液分离、纯化中性粒细胞,PCR检测中性粒细胞内HBV-DNA。结果慢性乙型肝炎患者治疗前中性粒细胞内HBV-DNA阳检率为30.95%(13/42),其中"大三阳"、"小三阳"患者中性粒细胞内HBV-DNA阳检率分别为52.94%(9/17)和16.00%(4/25);阿德福韦酯治疗后中性粒细胞内HBV-DNA阳检率为9.52%(4/42),其中"大三阳"、"小三阳"患者中性粒细胞内HBVDNA阳检率分别为17.65%(3/17)和4.00%(1/25),慢性乙型肝炎各组治疗前后阳检率相比均有统计学意义(P<0.05)。结论 HBV可感染患者中性粒细胞形成肝外隐匿感染;阿德福韦酯对潜伏或隐匿于中性粒细胞内的HBV具有较好的抑制作用。Objective To study the infection and replication of hepatitis B virus （HBV） in polymorphonuclear neutrophils of the patients with chronic hepatitis B. Methods The neutrophils were isolated and purified by neutrophil isolation medium, and the loads of HBV-DNA in neutrophUs were detected by PCR. Results Positive detection rate of HBV-DNA in neutrophils of chronic hepatitis B patients was 30.95% （13/42）, and among them, the HBeAg positive and HBeAg negative ones were 52.94% （9/17） and 16.00% （4/25）, respectively. After treatment of adefovir, the positive rate of HBV-DNA in neutrophils of chronic hepatitis B patients was 9.52% （4/42）, and among them, the HBeAg positive and HBeAg negative ones were 17.65% （3/17） and 4.00% （1/25）, respectively. There were significantly different positive rates in the patients before and after treatment （ P 〈 0.05）. Conclusion HBV can infect the neutrophils of the patients with chronic hepatitis B to achieve extrahepatic occult infection. Adefovir has a good inhibitory effect against the latent or hidden HBV in neutrophils.

关 键 词：中性粒细胞 中性粒细胞分离液 乙型肝炎病毒 感染 

分 类 号：R392.12[医药卫生—免疫学] R512.603[医药卫生—基础医学]