A high-sensitive detection method for carvone odor by implanted electrodes in rat olfactory bulb  

作　　者：Liujing Zhuang Ning Hu Feng Tian Qi Dong Liang Hu Rong Li Ping Wang 

机构地区：[1]Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry,Department of Biomedical Engineering, Zhejiang University

出　　处：《Chinese Science Bulletin》2014年第1期29-37,共9页

基　　金：supported by the National Key Basic Research Program of China(2009CB320303);the National Natural Science Foundation of China(81027003,60725102)

摘　　要：Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants.Taking advantages of cell culture and micro-fabrication technologies,olfactory cell-or tissue-based biosensor represent a promising platform for in vitro odorant detection.However,in vitro conditions lead to shortened cell/tissue survivals,and the working life of neuron chips is short.The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation.In this study,we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities.Then,we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains.The stable,repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks.Further,we explored the concentration detection sensitivity limitation of developed method,and found the detection low limit of carvone was below10-10mol/L.The result demonstrates that the concentration range of in vivo odorant detection method is much wider than in vitro method.Mammalian olfactory systems have extraordi- nary ability to sense and identify various trace odorants. Taking advantages of cell culture and micro-fabrication technologies, olfactory cell- or tissue-based biosensor represent a promising platform for in vitro odorant detec- tion. However, in vitro conditions lead to shortened cell/ tissue survivals, and the working life of neuron chips is short. The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation. In this study, we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities. Then, we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains. The stable, repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks. Further, we explored the concentration detection sensitivity limitation of developed method, and found the detection low limit of carvone was below 10-a~ mol/L. The result demonstrates that the concentra- tion range of in vivo odorant detection method is much wider than in vitro method.

关 键 词：检测灵敏度 阵列电极 香芹酮 气味 高灵敏度 植入 大鼠 神经元芯片 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] Q429.1[自动化与计算机技术—控制科学与工程]