EcR-RNAi和印楝素处理斜纹夜蛾幼虫诱导腹足发育受阻表型(英文)  

EcR-RNAi and azadirachtin treatments induced the abnormal proleg development in Spodoptera litura.

作　　者：赵锦程[1] 吴天旻 刘丽花[1] 汪洋[1] 何琳[1] 

机构地区：[1]华东师范大学生命科学学院,200241

出　　处：《华东师范大学学报（自然科学版）》2014年第1期133-142,共10页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(31201974,41376157);上海市科委基金(12ZR1408900);教育部博士点基金(20120076120011)

摘　　要：蜕皮激素是一种可以诱导一系列变态发育关键过程的昆虫蜕皮调节激素,与蜕皮激素受体结合以后与配体蛋白Usp作用形成一个复合体发挥作用.通过兼并引物克隆获得斜纹夜蛾一个600 bp的EcR cDNAs片段编码200个氨基酸,斜纹夜蛾是东亚地区一种主要的农作物害虫.利用这段序列设计了EcR-dsRNA通过注射幼虫的实验来分析EcR基因在不同发育阶段的功能.同时利用印楝素喂食RNAi注射的幼虫观察其与正常对照组有何发育差异.结果显示EcR RNAi会影响正常的蜕皮过程并导致不完成蛹化,在EcR RNAi注射并喂食印楝素的实验组,幼虫出现生长迟缓、发育进程紊乱和腹足发育不正常等现象,由此推测印楝素和EcR RNAi或许影响了蜕皮级联调控通路,由此引发变态发育过程中组织重建紊乱.Ecdysone is the insect molting hormone which induces a series of events critical for ecdysis, beginning with the formation of a complex between ecdysteroid receptor （EcR） and its partner protein, ultraspiracle （Usp）. We cloned a 600 bp partial cDNAs encoding 200 amino acids EcR protein by degenerate primers from the common cutworm, Spodoptera litura, a destructive agricultural pest insect in East Asia. We used this fragment to design the EcR-dsRNA to interfere the corresponding gene to analysis the change in different de- velopment stages. Using the Azadirachtin （Az） feeding the RNAi injected larvae to observe the development difference with normal control. Results indicated that the EcR RNAi in- terference the normal ecdysis and result in incomplete pupal molt. In the Az treatment after EcR RNAi injection, the larvae stunt growth and development program were disturbed and observed abnormal proleg phenotype. We deduced that Az and EcR RNAi maybe influence the key genes and inhibit the ecdysone cascade modulate pathway, which result in the dis- order of tissue remodeling in molting.

关 键 词：蜕皮激素受体 RNA干扰 印楝素 斜纹夜蛾 腹足表型 

分 类 号：Q965[生物学—昆虫学]