RANKL浓度对破骨细胞形成与活性的影响  

Effect of RANKL on formation and activity of osteoclasts

作　　者：李勇[1] 吴婷婷[2] 程豪[1] 谭鹏[1] 糜宝国[1] 张勇[1] 关邯峰[1] 熊伟[1] 廖晖[1] 陈安民[1] 李锋[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院骨科,武汉430030 [2]华中科技大学同济医学院附属同济医院儿科

出　　处：《骨科》2014年第1期5-8,共4页ORTHOPAEDICS

基　　金：国家自然科学基金(81271934);中央高校基本科研业务费专项资金(2013YGYL017);卫生部卫生行业科研专项项目(201002018)

摘　　要：目的探讨不同浓度RANKL对骨髓单核细胞向破骨细胞分化和活性的影响。方法 50、100和150μg/L三种RANKL浓度诱导骨髓单核细胞向破骨细胞分化。抗酒石酸酸性磷酸酶染色观察破骨细胞形态并计数,抗酒石酸酸性磷酸酶活性检测试剂盒检测上清中的抗酒石酸酸性磷酸酶活性,骨板吸收实验检测破骨细胞骨吸收活力。结果 100μg/L和150μg/L RANKL浓度组在破骨细胞数量、细胞大小、核数目,抗酒石酸酸性磷酸酶活性和骨吸收率方面均明显高于50μg/L组(P<0.01),而100μg/L和150μg/L组间没有明显差异。结论100μg/L RANKL浓度下破骨细胞形成和活性能力强且相对较经济,是破骨细胞诱导培养的理想浓度值。Objective To study the effects of RANKL on the formation and activity of osteoclasts. Methods RANKL at concentrations of 50, 100 and 150μg/L induced osteoclastogenesis from bone marrow mononuclear cells. Tartrate resistant acid phosphatase staining was used to observe the formation and morphology of osteo clasts. Tartrate resistant acid phosphatase assay kit was used to evaluate the tartrate resistant acid phosphatase ac- tivity in the culture supernatant. Bone absorption ability of osteoclats was evaluated by absorption assay of bone plate. Results The number, sizes, nucleus number, tartrate resistant acid phosphatase activity and bone absorp- tion ability of osteoclasts were enhanced in 100 μg/L and 150 μg/L RANKL groups as compared with 50μg/L RANKL group （P〈0. 01 ）. There was no significant difference between 100μg/L and 150 μg/L RANKL groups. Conclusion 100μg/L RANKL can enhance formation and activity of osteoclasts, and is an ideal concentration for osteoclast culture.

关 键 词：骨髓细胞 破骨细胞 核因子ΚB受体活化因子 配体 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]