3种药物及个人护理品对斜生栅藻生长及光系统Ⅱ的影响  

Effects of Three Pharmaceuticals and Personal Care Products on Growth and Photosystem Ⅱ in Scenedesmus obliquus

作　　者：朱术超[1] 刘滨扬[1] 陈本亮 张杰龙[1] 刘蔚秋[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]海珠湿地维护中心,广东广州510399

出　　处：《中山大学学报（自然科学版）》2014年第1期121-126,134,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(31070470,31370490);广东省科技计划资助项目(2012B030800013)

摘　　要：研究了乳糖酸红霉素（ETM）、双氯芬酸钠（DCF）及壬基酚十氧乙烯醚（NPEO10）等3种药物及个人护理品（PPCPs）对斜生栅藻（Scenedesmus obliquus）的生长及光系统II（PS II）的影响。结果表明：3种PPCPs对斜生栅藻的生长及其PSII均有抑制作用并以ETM的毒性最大，但NPEO10在低质量浓度时（0～0．05mg／L）对斜生栅藻的生长及PSII具一定的促进作用。对叶绿素荧光快速反应曲线的分析显示，ETM同时抑制斜生栅藻PSII反应中心的供体侧和受体侧，使PSII反应中心失活并降低光化学反应效率等，而DCF和NPEO10主要抑制电子传递链Q A-后的电子传递过程。研究显示叶绿素荧光诱导动力学参数对于反映PPCPs对藻类的毒性特征具有较高的敏感性及一定的特异性，可作为PPCPs污染生态风险评价的潜在候选生物标志物。The effects of three Pharmaceuticals and Personal Care Products （PPCPs）, namely erythromy- cin lactobionaten （ETM）, diclofenac sodium （DCF） and polyoxyethylene nonylphenol ethers （n = 10, NPEO10）, on growth and photoystem II （PS II） of Scenedesmus obliquus were investigated. The results showed that the three PPCPs could significantly decrease the growth rate and inhibit the PS II function of the tested algae, among which, ETM was the most toxic, followed by NPEO10 and DCF. However, low concentration （0. 05 mg/L） of NPEO10 could promote the growth rate and PS II function of S. obliquus. Analysis of chlorophyll fluorescence transient kinetics showed that ETM induced an inactivation of reaction center and inhibited both donor and acceptor sides of PS II reaction center, however, the other two PPCPs mainly inhibited the electron flow beyond Q~. Our study suggested that some chlorophyll fluorescence indices are highly sensitive and relatively specific to algal exposure of PPCPs, and may be potentially used as candidate biomarkers for the exposure of PPCPs.

关 键 词：药物及个人护理用品 斜生栅藻 IC50 快速叶绿素荧光诱导动力学 

分 类 号：Q89[生物学] X835[环境科学与工程—环境工程]