流式细胞仪液流聚焦系统仿真分析与设计  

Simulated analysis and design of flow cytometer focusing system

作　　者：马玉婷[1] 严心涛[1] 陈忠祥[1] 王策[1] 武晓东[1] 

机构地区：[1]中国科学院苏州生物医学工程技术研究所,苏州215163

出　　处：《分析仪器》2014年第1期17-22,共6页Analytical Instrumentation

基　　金：国家863基金资助项目"医用新型流式细胞仪研制"(2011AA02A106);中科院知识创新工程主要方向项目"新型流式细胞仪"(KGCX2-YW-912)

摘　　要：使用FLUENT对流式细胞仪液流系统中的核心部件流动室进行了流体仿真分析。探讨了样品流速、鞘液流速、进样针位置对聚焦效果的影响,分析了鞘液不对称进样的实施可能性,以及对鞘液脉动性的补偿措施。搭建了流式平台进行荧光微球的CV值测定,其变化趋势与仿真结果较为吻合。仿真结果为流式细胞仪液流系统的设计提供了参考依据。Simulated analysis on flow cell, which is core components of fluid system in flow cytometer, was conducted by use of FLUENT. The influence of sample flow rate, sheath flow rate, sample outlet po- sition on the focusing capability were discussed. The asymmetrical sheath inlet was also investigated, as well as the compensation of sheath pulsation. The experimental platform was built to test the CV of fluo- rescent microspheres. The changing of CV coincided with the simulated results. The simulated results provide a reference to the design of fluid system in flow cytometer.

关 键 词：流式细胞术 层流 聚焦 流动室 

分 类 号：TH79[机械工程—仪器科学与技术]